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当前位置: 首页 > 文章 > 猪ESR、FSHβ和leptin基因多态性及其与卵泡囊肿的相关性 吉林农业大学学报 2017 (2) 194-198+203
Position: Home > Articles > Single Nucleotide Polymorphisms of ESR,FSHβ and Leptin Genes in Porcine and Its Correlation with Follicular Cysts Journal of Jilin Agricultural University 2017 (2) 194-198+203

猪ESR、FSHβ和leptin基因多态性及其与卵泡囊肿的相关性

作  者：
王昊天;蒋彦文;高珊;郑雪;李纯锦;周虚
单  位：
吉林大学动物科学学院
关键词：
雌激素受体基因;促卵泡素β亚基基因;瘦素基因;母猪;卵泡囊肿;基因多态性
摘  要：
雌激素受体基因(ESR)、促卵泡素β亚基基因(FSHβ)和瘦素基因(leptin)是影响卵泡生长发育的主效基因,在母猪繁殖过程中起关键的作用。试验采用PCR和PCR-RFLP技术检测ESR、FSHβ和leptin基因的单核苷酸多态性,分析各基因多态性与卵泡囊肿的关系。结果表明:3个基因共检测到3个多态位点,分别是位于ESR基因内含子A>G突变,FSHβ基因内含子T>C突变,leptin基因外显子C>T突变。ESR的AA和AB基因型在健康群体和卵泡囊肿群体分布差异显著(P<0.05),该基因型可以作为检测卵泡囊肿的标志基因型;FSHβ基因和leptin基因的AA、AB和BB基因型分布在检测群体中均未见显著差异(P>0.05)。
译  名：
Single Nucleotide Polymorphisms of ESR,FSHβ and Leptin Genes in Porcine and Its Correlation with Follicular Cysts
作  者：
WANG Haotian;JIANG Yanwen;GAO Shan;ZHENG Xue;LI Chunjin;ZHOU Xu;College of Animal Sciences,Jilin University;
关键词：
ESR;;FSHβ;;leptin;;sow;;follicular cyst;;single nucleotide polymorphism
摘  要：
ESR,FSHβ and leptin are regarded as major genes affecting the growth of follicles. They play a key role in the process of sow reproduction. In this study,Polymerase Chain Reaction( PCR)and Restriction Fragment Length Polymorphism( RFLP) methods were used to detect single nucleotide polymorphisms of ESR,FSHβ and leptin genes. Then the association between genetic polymorphisms and follicular cysts was analyzed. The results showed that three polymorphic sites were detected within ESR,FSHβ and leptin genes,and they are ESR gene intron A>G mutation,FSHβ intron T>C mutation and leptin exon C>T mutation. The AA and AB genotype distributions of ESR gene in the sows with follicular cysts were significantly different( P < 0. 05) from those of the sows with no cysts. These genotypes can be used as marker genotypes to detect follicular cysts. The AA,AB and BB genotype distributions of FSHβ and leptin genes show no significant difference( P>0. 05) in normal and follicular cysts groups.

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