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当前位置: 首页 > 文章 > 鸡IL-18和IFN-γ协同抗病毒机制分析 安徽农学通报 2017 (11) 40-46
Position: Home > Articles > Synergistic Antiviral Mechanism of Chicken IL-18 and IFN-γ Anhui Agricultural Science Bulletin 2017 (11) 40-46

鸡IL-18和IFN-γ协同抗病毒机制分析

作  者：
李晓婷;宋佰芬;王歆桐;王梦瑶;余崴;马金柱;于立权;崔玉东
单  位：
黑龙江八一农垦大学动物科技学院;黑龙江八一农垦大学生命科学技术学院
关键词：
鸡白细胞介素18;鸡干扰素γ;抗病毒;免疫调节
摘  要：
禽病毒性疾病如禽传染性法氏囊病(Infectious Bursal Disease,IBD)一直困扰着养禽业的健康、快速发展。在其他抗病毒感染的药物效果不理想时,有必要应用细胞因子制剂来改善机体免疫并提高抗病毒能力。已证明,鸡白细胞介素18(Chicken interleukin-18,Ch IL-18)和鸡干扰素γ(Chicken interferon,Ch IFN-γ)对多种病毒感染具有抑制作用。为了研究新型细胞因子制剂,该实验分析了鸡白细胞介素18(Chicken interleu-kin-18,Ch IL-18)和鸡干扰素γ(Chicken interferon,Ch IFN-γ)以及二者混合后的抗病毒机制。Ch IL-18和Ch IFN-γ蛋白注射雏鸡后7d内,应用试剂盒检测雏鸡体内血清中细胞因子的变化情况。利用鸡外周血单核细胞分析Ch IL-18和Ch IFN-γ对细胞内NO、几种Toll样受体(Toll-like receptors,TLR)及下游信号通路的影响。实验结果表明,混合组蛋白可刺激雏鸡血清中产生高水平IL-6,IL-12,及IL-17。混合组蛋白可以诱导巨噬细胞中NO的分泌水平显著升高,TLR 2、TLR 3、TLR 4和MHC-II的表达量升高,MAPK和NFκB信号通路被激活。综上结果表明,Ch IL-18和Ch IFN-γ以及二者协同可诱导机体对病毒感染产生有效的防御作用,为新型细胞因子制剂的研究提供参考。
译  名：
Synergistic Antiviral Mechanism of Chicken IL-18 and IFN-γ
作  者：
Li Xiaoting;College of Animal Science and Technology,Heilongjiang Bayi Agricultural University;
关键词：
Chicken interleukin 18;;Chicken interferon γ;;Antiviral;;Immunoregulation
摘  要：
The healthy and rapid development of poultry industry was troubled by avian viral diseases such as Infec-tious Bursal Disease(IBD). With other anti-viral drug effect is not ideal,it is necessary to use cytokine preparationsto improve the immune system and improve the anti-virus ability. Chicken interleukin-18(Ch IL-18)and chickeninterferon gamma(Ch IFN-γ)have been shown to inhibit a variety of viral infections. In order to study the novel cyto-kine preparations,the effects of Ch IL-18 and Ch IFN-γ and the two mixed antiviral mechanism was analyzed. The re-sults of this laboratory had showed that Ch IL-18 and Ch IFN-γ can inhibit IBDV proliferation in chickens,especial-ly with the mixed group,which indicates that the mixed group has better antiviral effect. Within 7 days after Ch IL-18 and Ch IFN-γ injection of chickens,the kit was used to detect the changes of cytokines in the chicks. The effects ofCh IL-18 and Ch IFN-γ on intracellular NO,several Toll-like receptors(TLR)and downstream signaling pathwayswere analyzed by using peripheral blood mononuclear cells of chickens. The results showed that the levels of IL-6,IL-12 and IL-17 in the serum stimulated by mixed group ware significantly different from those in the PBS group.Mixed group proteins could induce NO production,the expression levels of TLR2,TLR3,TLR4 and MHC-II were in-creased and MAPK and NFκB signaling were activated. These results indicated that the mixed group had a better the-oretical basis for antiviral ability,Ch IL-18 and Ch IFN-γ,both of which could induce the body to produce effectivedefense against virus infection and provide reference for the study of new cytokine preparations.

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