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Position: Home > Articles > Study on Processing Antihypertensive Peptides Derived from Wheat Germ Protein FOOD SCIENCE 2003,24 (10) 120-123

从麦胚蛋白质中制备降血压肽的研究

作  者:
辛志宏;吴守一;马海乐;代春华
单  位:
江苏大学生物与环境工程学院
关键词:
麦胚蛋白;超临界CO2;HPCE;血管紧张素转化酶;降血压肽
摘  要:
用超临界CO2对小麦胚芽脱脂,碱溶酸沉淀法提取麦胚蛋白,用8种蛋白酶分别进行单一酶和复合酶水解麦胚蛋白生产降血压肽,用高效毛细管电泳(HPCE)测定其活性。碱性蛋白酶生产水解物的活性最大,其对麦胚蛋白作用的最适pH为9,温度为50~55℃,加酶量为24AU/kg,水解度为16.5%时所得水解物对ACE有强烈抑制用。
译  名:
Study on Processing Antihypertensive Peptides Derived from Wheat Germ Protein
作  者:
Xing Zhihong et al
关键词:
wheat germ protein;supercritical carbon dioxide;high performance capillary electrophoresis;angiotensoin-converting enzyme;antihypertensive peptides
摘  要:
After wheat germ was degreased by supercritical carbon dioxide ,the method of dissolving wheat germ protein byalkali and of deposition by acid was applied.The wheat germ protein was hydrolyzed by single and complex protease respec-tively in 8 kinds of proteases for antihypertensive peptide.Peptides activity was determined by high performance capillaryelectrophoresis. The hydrolyte obtained by alkaline protease showed the highest activity, with the optimum functionary pH as9 and temperature as 50~55℃,The quantity of protease used was 24AU/kg。

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