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当前位置: 首页 > 文章 > 1株杨梅炭疽病拮抗菌株的筛选与鉴定 贵州农业科学 2018 (11) 40-43
Position: Home > Articles > Isolation and Identification of a Antagonistic Strain Against Anthracnose in Morella rubra Guizhou Agricultural Sciences 2018 (11) 40-43

1株杨梅炭疽病拮抗菌株的筛选与鉴定

作  者：
卯婷婷;莫唯弟;赵玳琳;王廿;陶刚
单  位：
贵州省农业科学院植物保护研究所;贵州大学农学院
关键词：
杨梅;杨梅炭疽病;解磷真菌;拮抗菌株;生防菌
摘  要：
为杨梅炭疽病生物农药的开发提供新的生防菌资源,采用解磷选择性培养基分离及平板对峙筛选相结合的方法从杨梅种植土壤中筛选杨梅炭疽病菌拮抗菌株,通过菌落直径法验证其发酵液的抑菌特性,并利用形态学与分子生物学相结合的方法对拮抗菌株进行鉴定。结果表明:从杨梅根际土壤中分离获得52株解磷真菌,其中菌株YMMF150101对杨梅炭疽病菌有拮抗作用,平板对峙抑制率达81.6%;其发酵液及无菌发酵滤液均对靶标菌生长有抑制作用,抑制率分别达84.73%和52.53%;根据形态学特征结合其分子系统发育学鉴定,YMMF150101为米曲霉菌(Aspergillus oryzae)。
译  名：
Isolation and Identification of a Antagonistic Strain Against Anthracnose in Morella rubra
作  者：
MAO Tingting;MO Weidi;ZHAO Dailin;WANG Nian;TAO Gang;Institute of Plant Protection,Guizhou Academy of Agricultural Sciences;College of Agriculture,Guizhou University;
单  位：
MAO Tingting%MO Weidi%ZHAO Dailin%WANG Nian%TAO Gang%Institute of Plant Protection,Guizhou Academy of Agricultural Sciences%College of Agriculture,Guizhou University
关键词：
Morella rubra;;anthracnose;;phosphate-solubilizing fungus;;antagonistic strain;;bio-control fungi
摘  要：
An antagonistic strain against anthracnose was isolated from Morella rubra planting soil by culture of phosphate-solubilizing selective medium and plate confrontation,the inhibition characteristics of the fermentation broth of the antagonistic strain was tested by the colony diameter method and the isolated antagonistic strain was identified by the methods of morphology and molecular biology to provide the new bio-control fungi for development of biopesticides against anthracnose in M.rubra.Result:52 phosphatesolubilizing fungi strains are isolated from rhizosphere soil of M.rubra and the plate confrontation inhibition rate of YMMF150101 strain with an antagonism against M.rubraanthracnose reaches 81.6%.The inhibition rate of its fermentation broth and sterile fermentation filtrate against growth of target fungi is 84.73% and 52.53% respectively.YMMF150101 strain is identified as Aspergillus oryzae by its morphological characteristics and molecular phylogeny.

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