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Position: Home > Articles > Molecular variation of Sweet potato feathery mottle virus coat protein gene Plant Protection 2012,38 (2) 114-116+123

甘薯羽状斑驳病毒外壳蛋白基因的分子变异

作  者:
王晓华;张振臣;乔奇;秦艳红;张德胜;田雨婷
单  位:
河南省农业科学院植物保护研究所
关键词:
甘薯羽状斑驳病毒;外壳蛋白基因;分子变异;SSCP分析
摘  要:
应用单链构象多态性(single-strand conformation polymorphism,SSCP)技术结合核苷酸序列测定的方法,对我国甘薯主产区11个省份的甘薯羽状斑驳病毒(Sweet potato feathery mottle virus,SPFMV)外壳蛋白(CP)基因的分子变异情况进行了研究。结果表明,SPFMV CP基因的RT-PCR产物表现了较丰富的图谱类型,50个分离物共产生9种主要的SSCP带型;对显示不同带型的20个样品的CP基因进行了序列测定和进化树分析,CP基因核苷酸序列一致性为77.2%~99.9%。说明这些样品的SPFMV的CP基因存在较大的分子变异,可划分为EA、RC、O和C 4个株系。
译  名:
Molecular variation of Sweet potato feathery mottle virus coat protein gene
作  者:
Wang Xiaohua1,Zhang Zhenchen1,2,Qiao Qi1,2,Qin Yanhong1,2,Zhang Desheng1,2,Tian Yuting1,2 (1.Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China; 2.Henan Key Laboratory of Crop Pest Control,Zhengzhou 450002,China)
关键词:
SPFMV;coat protein gene;molecular variation;SSCP analysis
摘  要:
Virus samples were collected from 11 provinces in China,and the molecular variation of Sweet potato feathery mottle virus(SPFMV)coat protein(CP) gene were analyzed using single-strand conformation polymorphism(SSCP) and sequence analysis.SSCP analysis of 50 isolates showed that these isolates produced 9 kinds of SSCP band forms.The results of sequence comparison and phylogenetic analysis showed that the identities of the nucleotide sequences of CP genes among these isolates were 77.2%-99.9%.SPFMV CP genes of different isolates in China had great molecular variability and could be divided into four diverged groups at least,including the East Africa(EA),Russet Crack(RC),Ordinary(O) and Common(C) strain groups.

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