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当前位置: 首页 > 文章 > 枯草芽胞杆菌NIP3产伊枯草菌素发酵条件优化及对柑橘青霉病的防效 中国农学通报 2024,40 (35) 26-34
Position: Home > Articles > Optimization of Fermentation Conditions for Production of Iturin by Bacillus subtilis NIP3 and Its Control Effect on Citrus Blue Mold Chinese Agricultural Science Bulletin 2024,40 (35) 26-34

枯草芽胞杆菌NIP3产伊枯草菌素发酵条件优化及对柑橘青霉病的防效

作  者：
张悦;齐晓华;徐明玉;杜春梅
单  位：
黑龙江大学
关键词：
柑橘青霉病;枯草芽胞杆菌;伊枯草菌素;响应面优化;防效
摘  要：
柑橘青霉病是柑橘类水果储运期的常发病害,造成的经济损失严重.伊枯草菌素具有防治该病害的潜力,但是产量较低.本研究对枯草芽胞杆菌NIP3产生伊枯草菌素的3个关键因素进行了响应面优化,并分析了伊枯草菌素在体内和体外对意大利青霉的防治效果.响应面分析结果表明,枯草芽胞杆菌NIP3产生伊枯草菌素的最佳葡萄糖浓度为30 g/L、L-谷氨酸钠浓度为6 g/L、温度为33℃.在此条件下,伊枯草菌素产量达到721.33±3.72 mg/L,较优化前提高了51.59%.检测方法对伊枯草菌素的体外防治效果有显著的影响.利用杯碟法测得伊枯草菌素对意大利青霉生长的最低有效浓度为312.5 μg/mL.而利用凹玻片法和菌丝体干重法测得的伊枯草菌素抑制意大利青霉孢子萌发和菌丝体生长的有效中浓度(EC50)分别为29.11 μg/mL和22.80 μg/mL.果实体内防效试验表明,10 mg/mL的伊枯草菌素对柑橘青霉病的预防效果好于治疗效果,且发病抑制率可以达到100%.本研究为开发柑橘青霉病生物防治剂提供了科学依据.
译  名：
Optimization of Fermentation Conditions for Production of Iturin by Bacillus subtilis NIP3 and Its Control Effect on Citrus Blue Mold
关键词：
citrus blue mold disease%Bacillus subtilis%iturin%response surface optimization%control effect
摘  要：
Citrus blue mold is a prevalent disease affecting citrus fruits during storage and transportation,leading to serious economic losses.Iturins exhibit potential for controlling citrus blue mold disease,but its yield remains low.This study conducted the response surface optimization on 3 key factors influencing the production of iturins by Bacillus subtilis NIP3,and analyzed the efficacy of iturins against Penicillium italicum both in vivo and in vitro.The results from response surface analysis showed that the optimal concentrations of glucose and L-glutamate,as well as temperature,were 30 g/L,6 g/L,and 33℃ respectively for maximizing iturin production by B.subtilis NIP3.Under these optimized conditions,the yield of iturins reached 721.33±3.72 mg/L,an increase of 51.59%compared to pre-optimization levels.The detection method significantly influenced the control efficacy of iturins in vitro.The Oxford cup diffusion method revealed that the lowest effective concentration of iturins inhibiting P.italicum growth was 312.5 μg/mL.Furthermore,the effective concentration(EC50)inhibiting spore germination and mycelium growth were determined to be 29.11 μg/mL and 22.80 μg/mL respectively when measured via hollow slide method and mycelium dry weight method.Results demonstrated that while at a concentration of 10 mg/mL,iturins exhibited superior preventive effects over therapeutic effects against citrus penicilliosis,with an incidence inhibition rate reaching up to 100%.This study provides a scientific foundation for developing biocontrol agents targeting citrus blue mold disease.

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