当前位置: 首页 > 文章 > 王草根际解磷菌的分离及解磷能力测定 广东农业科学 2014,41 (10) 69-72
Position: Home > Articles > Isolation and phosphate solubilizing capacity determination of rhizosphere phosphate-solubilizing microorganism in King grass Guangdong Agricultural Sciences 2014,41 (10) 69-72

王草根际解磷菌的分离及解磷能力测定

作  者:
赵艳;阮云泽;张晓波
单  位:
海南大学农学院
关键词:
王草;根际;解磷菌;解磷能力
摘  要:
选用蒙金娜有机培养基,从王草根际分离出21株具有溶磷能力的菌株,利用溶磷圈法和钼锑抗比色法对其解磷能力进行测定。结果显示,21株菌株在蒙金娜有机磷培养基上均形成明显透明圈,其培养48 h D/d值在1.3~7.0之间,72 h D/d值在1.72~5.5之间,120 h D/d值在1.33~4.67之间;用钼锑抗比色法测定溶磷量为4.11~327.1 mg/mL。经解磷能力分析,菌株2-6、2-7-1、2-9、4-4具有较强的解磷能力,这些菌株在后续微生物菌肥研制中具有较大潜力。
译  名:
Isolation and phosphate solubilizing capacity determination of rhizosphere phosphate-solubilizing microorganism in King grass
作  者:
ZHAO Yan;RUAN Yun-ze;ZHANG Xiao-bo;College of Agriculture,Hainan University;Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresource,Hainan University;
关键词:
King grass;;rhizosphere;;phosphate-solubilizing microorganism;;phosphate dissolving capacity
摘  要:
Phosphate-solubilizing microorganisms were isolated from King grass rhizospheres using Mengjinna organic culture media.The phosphate solubilizing capacities were investigated from 41 strains based on phosphate-solubilizing circle and molybdenum blue method.The results indicated that 21 strains had ability to solubilize organic phosphate on Mengjinna organic culture medium and 48 h D/d(diameter of phosphate-dissolving zone/diameter of bacterial clone) value was from 1.3to 7.0; 72 h D/d value was from 1.72 to 5.5; 120 h D/d value was from 1.33 to 4.67.In liquid culture,the available phosphate increment was from 4.11 to 327.1 mg/mL using molybdenum blue method by inorganic phosphate-solubilizing microorganism.Strains 2-6,2-7-1,2-9 and 4-4 had higher ability of organic phosphate dissolving,showed great potential for agricultural use in microbiological fertilizer.

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