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Position: Home > Articles > Study on the Separation and Purification of Total Flavonoids from Sedum aizoon L. Journal of Anhui Agricultural Sciences 2017 (18) 113-114+160

费菜总黄酮分离纯化方法研究

作  者：
薛志忠;杨雅华;张国新;张薇
单  位：
河北省农林科学院滨海农业研究所;河北省盐碱地绿化工程技术研究中心
关键词：
费菜;总黄酮;聚合物纳米微球;分离纯化
摘  要：
[目的]开发高纯度费菜总黄酮的分离纯化工艺。[方法]以大孔树脂为载体,对上样量及层析条件进行优化,再进一步采用结晶的方法进行纯化。[结果]选用聚合物纳米微球PS RPC-300作为最佳层析填料,洗脱速度为5 m L/min,洗脱剂为70%乙醇,上样量为5.0 g/kg填料,结晶溶剂为丙酮;在此优化条件下所得产品的纯度为95.1%。[结论]此分离纯化方法简便可靠,分离效果好。
译  名：
Study on the Separation and Purification of Total Flavonoids from Sedum aizoon L.
作  者：
XUE Zhi-zhong;YANG Ya-hua;ZHANG Guo-xin;Coastal Agriculture Institute,Hebei Academy of Agriculture and Forestry Sciences;Hebei Engineering Research Center for Saline-alkali Land Greening;
关键词：
Sedum aizoon L.;;Total flavonoids;;Polymer nanospheres;;Separation and purification
摘  要：
[Objective] The research aimed to develop the separation and purification processes of high-purity total flavonoids from Sedum aizoon L.[Method]With the macroporous resin as the carrier,the loading volume and the chromatographic conditions were optimized,and then further purified by crystallization method.[Result]Type of PS RPC-300 nanoscale polymer particles was selected as the best chromatography filler. The optimal conditions were as follows: the elution speed was 5. 0 m L/min,70% ethanol was used as the eluant,the amount of sample was 5 g/kg filter,and acetone was used as the crystallization solvent. The purity of the final product was 95. 1% under the optimal conditions.[Conclusion]The separation and purification method is simple and reliable,and the separation effect is good.

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