当前位置: 首页 > 文章 > ‘品丽珠’葡萄离体茎尖超低温保存的研究 园艺学报 2001,28 (1) 62-64
Position: Home > Articles > Study on Cryopreservation of in Vitro Cultured Cabernet France Shoot Tips Acta Horticulturae Sinica 2001,28 (1) 62-64

‘品丽珠’葡萄离体茎尖超低温保存的研究

作  者:
赵艳华;吴永杰
单  位:
河北省农林科学院昌黎果树研究所
关键词:
葡萄;茎尖;超低温
摘  要:
研究了包埋干燥法超低温保存‘品丽珠’葡萄离体茎尖的影响因素———芽的位置、干燥时间、预冰冻温度及材料生理状态。选继代培养 4个月以上的侧芽 ,蔗糖预培养3d、干燥 5h ,两步降温和直接在MS培养基上培养 ,存活率达 4 0 %。
译  名:
Study on Cryopreservation of in Vitro Cultured Cabernet France Shoot Tips
作  者:
Zhao Yanhua and Wu Yongjie (Changli Institute of Pomology,Hebei Acadimic of Agriculture and Forestry Sciences Institute,Changli 066600)
关键词:
Grape;Cryopreservation;Shoot tips
摘  要:
Bud location,dehydration,prefreezing and subculture time were studied on grapevine Cabernet France shoot tip cryopreservation by the encapsulation dehydration process.Best result(40%)was obtained in the following procedare:70 select lateral buds after four months’subculture as explants,preculture them for 3 days,dehdrate for five hours then cool them in two steps and finally subculture them on standard culture medium.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊