当前位置: 首页 > 文章 > 培养基中无机盐浓度及培养方式对栀子组培苗增殖生长的影响 中国农学通报 2014,30 (13) 256-259
Position: Home > Articles > Effect of Inorganic Salts Concentrations in Medium and Culture Methods on Proliferation and Growth of Gardenia jasminoides Ellis Chinese Agricultural Science Bulletin 2014,30 (13) 256-259

培养基中无机盐浓度及培养方式对栀子组培苗增殖生长的影响

作  者:
魏楠楠;朴炫春;李阳;廉美兰
单  位:
延边大学农学院
关键词:
栀子;组织培养;增殖生长;生物反应器
摘  要:
以栀子(Gardenia jasminoides Ellis)组培苗作为试验材料,研究培养基中无机盐浓度及培养方式对栀子组培苗增殖的影响,为栀子的大规模生产和快速繁殖提供理论依据。试验结果表明:MS培养基适合栀子的增殖培养;栀子增殖培养时总氮浓度以60 mmol/L为宜;NO3-/NH4+为40 mmol/L/20 mmol/L有利于栀子不定苗的增殖;反应器间歇浸没培养为最适宜的培养方式。
译  名:
Effect of Inorganic Salts Concentrations in Medium and Culture Methods on Proliferation and Growth of Gardenia jasminoides Ellis
作  者:
Wei Nannan;Piao Xuanchun;Li Yang;Lian Meilan;Agriculture of Yanbian University;
关键词:
Gardenia jasminoides Ellis;;tissue culture;;proliferation and growth;;bioreactor
摘  要:
The study was conducted to investigate the effect of inorganic salts concentrations and culturemethods on the proliferation of Gardenia jasminoides, in order to supply theoretical bases for large-scale andrapid production. Gardenia jasminoides plantlets in vitro were used as experiment materials. The resultsshowed that the standard MS strength(1.0 MS) was optimal. In addition, 60 mmol/L nitrogen and 40 mmol/LNO3-mixed 20 mmol/L NH4+in medium were the most appropriate for proliferation of Gardenia jasminoidesplantlets in vitro. Ebb and flood bioreactor culture was the most suitable culture method.

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