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Position: Home > Articles > Obtaining and Identification of Tetraploid Brassica nigra Acta Horticulturae Sinica 2015,42 (12) 2505-2511

四倍体黑芥的获得与鉴定

作  者:
张巍巍;刘富强;轩淑欣;冯大领;张成合;申书兴
单  位:
河北农业大学生命科学学院;河北农业大学园艺学院;保定市农业科学研究院
关键词:
黑芥;四倍体;秋水仙素
摘  要:
以黑芥为材料进行了同源多倍体的诱导和鉴定研究。结果表明,用0.1%秋水仙素药液处理黑芥籽苗生长点24~72 h均可诱导获得多倍体植株,其中处理48 h的诱导效果最好,四倍体株率达73.33%。四倍体黑芥长势良好,叶片和花变大,叶柄变短,叶色和花色变深,开花期较二倍体晚2~3 d。四倍体黑芥能正常开花散粉,但结籽率较低,仅为13.83%。
译  名:
Obtaining and Identification of Tetraploid Brassica nigra
作  者:
ZHANG Wei-wei;LIU Fu-qiang;XUAN Shu-xin;FENG Da-ling;ZHANG Cheng-he;SHEN Shu-xing;College of Life Science,Agricultural University of Hebei;Baoding Academy of Agricultural Sciences;College of Horticulture,Agricultural University of Hebei;
关键词:
Brassica nigra;;autotetraploid;;colchicine
摘  要:
Inducing tetraploid plants is an effective way to overcome the infertility of distant hybridization. In the present paper,tetraploid inducing and identification of Brassica nigra were conducted. The results showed that treating the seedling apexes of B. nigra with 0.1% colchicine solution for 24–72 h,the tetraploid plants were induced,in which the treatment for 48 hours achieved the highest percentage(73.33%)of tetraploid plants. The tetraploid plants grew and developed normally,their blades and flowers were obviously larger than those of diploid plants. The anthesis of the tetraploid plants was about 2–3 days later than the diploid plants. The seed setting of tetraploid plants was only 13.83%.

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