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健康屠宰猪中博卡病毒的检测及遗传演化分析

作  者:
张志;王赛赛;李岚;刘爽;李蕾;郑辉;吴发兴;张燕霞;李晓成
单  位:
福建农林大学;云南农业大学;中国动物卫生与流行病学中心;东北农业大学
关键词:
猪博卡病毒;套式PCR;遗传演化;健康猪
摘  要:
为了解我国健康猪群中猪博卡病毒(PBoV)的感染状况,从屠宰场内采集猪淋巴结、脾脏和扁桃体等组织,分别采用套式PCR进行猪博卡病毒1型和2型的检测。结果表明,28份样品中有2份样品用猪博卡病毒2型引物可扩增出439 bp的特异性条带;结构蛋白VP1基因序列分析表明,这2份样品均为猪博卡病毒2型感染,且其VP1序列与猪博卡病毒参考株HM053694的同源性最高,分别达93.8%和93.3%,同属PBoV1/PBoV2进化分支;而与另一个猪博卡病毒V6/V7进化分支的同源性仅为35.1%~39.2%,距离较远。
译  名:
Detection and genetic analysis of bocavirus in healthy swine
作  者:
ZHANG Zhi1,WANG Sai-sai2,LI Lan3,LIU Shuang1,LI Lei4, ZHENG Hui1,WU Fa-xing1,ZHANG Yan-xia1,LI Xiao-cheng1(1.China Animal Health and Epidemiology Center,Qingdao 266032,China; 2.Fujian Agriculture and Forestry University,Fuzhou 350002,China; 3.Yunnan Agricultural University,Kunming 650201,China; 4.Northeast Agricultural University,Harbin 150030,China)
关键词:
porcine bocavirus;nested RT-PCR;genetic analysis;healthy pigs
摘  要:
Lymphoid organ,spleen and tonsil from pigs in a slaughter farm were collected for detection of bocavirus by nested RT-PCR.The result showed that two were infected with porcine bocavirus type 2(PBoV2) in 28 samples.The VP1 sequence analysis indicated that the homologies of these two strains were 93.8% and 93.3% with porcine bocavirus strain HM053694 in V1/V2 cluster,but 35.1%~39.2% with V6/V7 cluster,which indicates a close relationship with PBoV1/PBoV2 subgroup.

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