中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 反相高效液相色谱法检测奶粉中含硫氨基酸 食品科学 2012,33 (8) 167-170
Position: Home > Articles > Determination of Sulfur-Containing Amino Acids in Milk Powder by Reverse-Phase High Performance Liquid Chromatography FOOD SCIENCE 2012,33 (8) 167-170

反相高效液相色谱法检测奶粉中含硫氨基酸

作  者：
李玉玲;李卫华;杨秀清
单  位：
山西大学生物技术研究所山西出入境检验检疫局;山西出入境检验检疫局;河南农业大学牧医工程学院
关键词：
奶粉;乳蛋白;含硫氨基酸;高效液相色谱法
摘  要：
目的:建立奶粉中含硫氨基酸反相高效液相色谱检测方法,为含硫氨基酸检测提供新思路。方法:用常规酸水解和氧化水解法水解奶粉,对水解条件和色谱分离和检测条件进行研究,用反相高效液相色谱法测定奶粉中的含硫氨基酸。结果:蛋氨酸(Met)、胱氨酸[(Cys)2]检出限分别为0.7、0.3μmol/L,定量检测范围均为10～300μmol/L,相关系数分别为0.9993、0.9992。经酸水解和氧化水解处理的奶粉,所测得的Met含量无显著差异,均为0.43g/100g,(Cys)2的含量则分别为0.06、0.14g/100g,差异显著。结论:奶粉中Met含量的高效液相色谱HPLC检测可选用酸水解或氧化水解中任一种前处理方法,(Cys)2含量的测定只能采用氧化法进行前处理。
译  名：
Determination of Sulfur-Containing Amino Acids in Milk Powder by Reverse-Phase High Performance Liquid Chromatography
作  者：
LI Yu-ling1,LI Wei-hua2,,YANG Xiu-qing1(1.Bio-technology Institute,Shanxi University,Taiyuan 030006,China; 2.Shanxi Entry-Exit Inspection and Quarantine Bureau,Taiyuan 030024,China)
关键词：
milk powder;lactoprotein;sulfur amino acids;high performance liquid chromatography
摘  要：
Objective: To establish a new method for the determination of sulfur-containing amino acids in milk powder by reverse-phase high performance liquid chromatography(HPLC).Methods: Milk powder was subjected to acid hydrolysis or oxidation and subsequent hydrolysis.The optimal hydrolysis conditions and chromatographic separation conditions were investigated.Results: The detection limits of the method for methionine and(Cys)2 were 0.7μmol/L and 0.3μmol/L,respectively,and the linear ranges were both 10-300μmol/L with correlation coefficients of 0.9993 and 0.9992,respectively.In addition,no significant difference was observed between the results of methionine content obtained with acid hydrolysis and oxidation and subsequent hydrolysis,which were both 0.43 g/100 g,whereas the(Cys)2 contents were 0.06 g/100 g and 0.15 g/100 g,respectively,showing a significant difference.Conclusion: Both acid hydrolysis and oxidation followed by hydrolysis can be used for the detection of methionine in milk powder,but only the latter is suitable for the detection of(Cys)2.

相似文章

导出引用
计量
文章访问数: 5
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46