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Progress in Veterinary Medicine
2012,33
(10)
9-13
西藏当雄县无浆体分子流行病学调查
作 者：
孙彩琴;刘建枝;黄磊;罗布顿珠;汪月凤;刘志杰;罗建勋;殷宏
单 位：
西藏自治区农牧科学院畜牧兽医研究所;西藏大学农牧学院;中国农业科学院兰州兽医研究所
关键词：
羊无浆体;16S rRNA基因;西藏革蜱;银盾革蜱
摘 要：
为了调查我国西藏地区无浆体的分布和流行情况,2011年5月从西藏当雄县采集蜱样511份,其中西藏革蜱(Dermacentor everestianus)66份、银盾革蜱(Dermacentor niveus)445份,以16SrRNA基因为检测目标,采用无浆体属特异性引物EE1/EE2对蜱的全基因组进行PCR扩增,对PCR阳性样本进行随机克隆、测序,经过序列比对和系统发育分析,结果发现这些阳性样品均为一种无浆体病原——羊无浆体(Anaplasma ovis)感染,且它们与在甘肃发现的无浆体具有较近的亲缘关系。PCR结果显示,这些蜱的总感染率为6.7%(34/511)。
译 名：
Molecular Prevalence of Anaplasma ovis in Dangxiong County of China Tibet
作 者：
SNU Cai-qin1,LIU Jian-zhi2,HUANG Lei1,LUOBU Dun-zhu3,WANG Yue-feng1,LIU Zhi-jie1,LUO Jian-xun1,YIN Hong1(1.State Key Laboratory of Veterinary Etiological Biology,Key Laboratory of Animal Parasitology of Gansu Province,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China;2.Tibet Livestock Research Institute,Tibet Academy of Agricultural and AnimalHusbandry Sciences,Lasa,Tibet,850009,China;3.Agricultural and Animal Husbandry faculty of Tibet University,Linzhi,Tibet,860000,China)
关键词：
Anaplasma ovis;16 S rRNA gene;Dermacentor everestianus;Dermacentor niveus
摘 要：
In order to investigate the prevalence of Anaplasma spp.in Tibetan area,511 adult ticks,66 Dermacentor everestianus and 445 Dermacentor niveus respectively,were collected from Dangxiong County in May 2011.All the tick DNA samples were detected by PCR targeting a 1 430 bp fragment of 16 S rRNA gene of Anaplasma spp.with the primer set EE1/EE2.The pairwise similarities and phylogenetic relationship were analyzed based on the partial 16 S rRNA sequences.Sequencing results indicated that only Anaplasma ovis was present,and its incidence was 6.7%(34/511).
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