中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 重组毕赤酵母生产耐高温α-淀粉酶发酵条件的优化 食品科学 2011,32 (23) 266-269
Position: Home > Articles > Optimization of Fermentation Conditions for Thermostable α-Amylase Production by Recombinant Pichia pastoris FOOD SCIENCE 2011,32 (23) 266-269

重组毕赤酵母生产耐高温α-淀粉酶发酵条件的优化

作  者：
秦亚楠;孟宪梅;潘风光;孙肖明;李凤祥;石彦忠
单  位：
吉林大学军需科技学院;吉林工商学院粮油食品深加工重点实验室
关键词：
毕赤酵母;耐高温α-淀粉酶;发酵;优化
摘  要：
利用重组毕赤酵母诱导表达可产生耐高温α-淀粉酶,在摇瓶发酵基础上,从诱导时间、甲醇添加量、pH值、接种量4个因素考察发酵条件对耐高温α-淀粉酶酶活力的影响,结合四元线性回归正交试验设计研究方法构建出甲醇诱导重组毕赤酵母生产耐高温α-淀粉酶的回归模型;优化最佳工艺参数为:诱导时间96h、甲醇添加量0.5%、接种量150mL/100mL、摇床转速200r/min。确定最优工艺条件下,耐高温α-淀粉酶酶活为217.2U/mL。
译  名：
Optimization of Fermentation Conditions for Thermostable α-Amylase Production by Recombinant Pichia pastoris
作  者：
QIN Ya-nan1,2,MENG Xian-mei1,,PAN Feng-guang2,,SUN Xiao-ming1,LI Feng-xiang1,SHI Yan-zhong1(1.Key Laboratory of Oil and Foodstuffs Processing,Jilin Business and Technology College,Changchun 130062,China; 2.College of Quartermaster Technology,Jilin University,Changchun 130062,China)
关键词：
Pichia pastoris;thermostable α-amylase;fermentation;optimization
摘  要：
The methanol-induced expression of thermostableα-amylase in a recombinant Pichia pastoris strain with the thermostableα-amylase gene of Bacillus licheniformis that had been constructed in our previous work was investigated during shake flask cultivation.A 4-variable linear regression design was used to explore the effects of 4 main cultivation conditions such as induction time,methanol amount,pH and inoculation amount on the expressed activity of thermostableα-amylase.A regression model describing the relationship between them was established.It was found that the optimal fermentation conditions were 96 h cultivation at an inoculation amount of 150 mL/100mL and a shake speed of 200 r/min in the presence of 0.5% methanol,resulting in a thermostableα-amylase activity of 217.2 U/mL.

相似文章

导出引用
计量
文章访问数: 14
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46