中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 从毕赤酵母中提取外源重组蛋白的超声破碎条件及优化 食品科学 2012,33 (10) 57-60
Position: Home > Articles > Optimization of Ultrasonic Treatment for Recovery of Recombinant Protein from Pichia pastoris FOOD SCIENCE 2012,33 (10) 57-60

从毕赤酵母中提取外源重组蛋白的超声破碎条件及优化

作  者：
戴佳锟;李燕;马齐;关正君;张超;张艳婷;赵荣荣;尉亚辉
单  位：
陕西省酶工程技术中心;西北大学生命科学学院,西部资源生物与现代生物技术省部共建重点实验室;陕西省科学院酶工程研究所;陕西省微生物研究所
关键词：
毕赤酵母;超声破碎法;人脂联素蛋白;Western Blotting免疫印记技术
摘  要：
建立适用于从毕赤酵母菌中提取外源重组蛋白的超声破碎方法。采用Western Blotting免疫印记技术检测从毕赤酵母菌中提取的外源人脂联素蛋白活性,并以外源人脂联素蛋白活性作为考察指标,采用单因素和L9(33)正交试验设计,对超声破碎条件进行优化。结果表明:在外源人脂联素蛋白活性最高的情况下,超声破碎条件为超声破碎功率450W、时间25min、时间间隔(工作时间:间歇时间)10:10(s/s),此时细胞破碎率为(67.8±2.1)%。若在超声破碎时添加1mmol/L PMSF,虽对细胞破碎率并无显著影响,但外源人脂联素蛋白活性将会明显增高。
译  名：
Optimization of Ultrasonic Treatment for Recovery of Recombinant Protein from Pichia pastoris
作  者：
DAI Jia-kun 1,2,3,LI Yan 4,MA Qi 1,2,GUAN Zheng-jun 3,ZHANG Chao 3,ZHANG Yan-ting 3,ZHAO Rong-rong 3,WEI Ya-hui 3,(1.Institute of Enzyme Engineering,Shaanxi Academy of Sciences,Xi,an 710600,China;2.Enzyme Engineering Technology Center of Shaanxi,Xi,an 710600,China;3.Key Laboratory of Resource Biology and Biotechnology in Western China,College of Life Science,Northwest University,Xi,an 710069,China;4.Shaanxi Provincial Institute of Microbiology,Xi,an 710043,China)
关键词：
Pichia pastoris;ultrasonication;human-adiponectin protein;Western Blotting analysis
摘  要：
In order to establish an appropriate ultrasonic treatment method for the recovery of recombinant protein from Pichia pastoris,the activity of human-adiponectin recombinant protein was evaluated by Western Blotting analysis.A L9(33) orthogonal array design based on single-factor tests was employed to optimize the conditions for ultrasonic treatment.The results showed that the optimal ultrasonic treatment conditions were ultrasonic power of 450 W,ultrasonic treatment time of 25 min and ultrasonic treatment interval of 10 s.Under the optimal ultrasonic treatment conditions,the breaking rate of cell walls was(67.8 ± 2.1)%.The activity of human-adiponectin recombinant protein could be significantly improved by adding 1 mmol/L of PMSF although the addition of PMSF had no effect on breaking rate of cell walls.

相似文章

导出引用
计量
文章访问数: 25
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46