Position: Home > Articles > Microfloral Changes of Vacuum Packaged Venison during Chilled Storage
MEAT RESEARCH
2015
(4)
15-19
真空包装冷却鹿肉贮藏过程中的菌相变化
作 者：
施荷;胡铁军;秦凤贤;陈巍;刘晶;王朝晖;贾冬舒;武军;尤丽新
单 位：
吉林省东鳌鹿业科技开发有限公司;吉林农业大学食品科学与工程学院;吉林省鹿产业工程研究中心;长春科技学院生物食品学院;延边大学农学院;吉林省出入境检验检疫局
关键词：
冷却鹿肉;真空包装;菌相变化;聚合酶链式反应-变性梯度凝胶电泳
摘 要：
利用聚合酶链式反应-变性梯度凝胶电泳方法研究真空包装的冷却鹿肉贮藏过程中的菌相变化。将冷却鹿肉真空包装贮藏于0~2℃条件下,每隔5 d取出,利用细菌试剂盒提取细菌DNA,进行聚合酶链式反应-变性梯度凝胶电泳分析,同时对16S rDNA V3区的DGGE图谱进行割胶测序,检测到贮藏末期主要是苏黎世克罗诺杆菌、斯氏普罗威登斯菌,棕色类香味菌,乳酸发酵类梭菌等成为优势腐败菌。
译 名：
Microfloral Changes of Vacuum Packaged Venison during Chilled Storage
作 者：
SHI He;HU Tiejun;QIN Fengxian;CHEN Wei;LIU Jing;WANG Zhaohui;JIA Dongshu;WU Jun;YOU Lixin;CHEN Haiyan;YU Yan;LIU Fang;MA Jinxi;YANG Bin;ZHANG Tiehua;College of Agriculture,Yanbian University;Jilin Dongao Deer Industry Science and Technology Development Co.Ltd.;Engineering Research Center of Deer Industry of Jilin Province;Jilin Entry-Exit Inspection and Quarantine Bureau;College of Food Science and Engineering,Jilin Agricultural University;College of Organism and Food,Changchun University of Science and Technology;College of Quartermaster Technology,Jilin University;
关键词：
chilled venison;;vacuum packaging;;microfloral change;;PCR-DGGE
摘 要：
The microfloral changes of vacuum packaged venison during chilled storage were examined using polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE).Samples were collected at 5-day intervals during the storage for bacterial DNA extraction using a DNA extraction kit before PCR-DGGE analysis.The band of 16 SrDNAV3 region from the DGGE analysis was excised for sequence analysis.The dominant spoilage bacteria at the end of the Storage included Cronobacter turicensis,Providencia stuartii,Myroides phaeus,Clostridium lactatifermentans.
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