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Position: Home > Articles > Cloning of Transcription Factor DREB1A Gene and Construction of Plant Expression Vector Journal of Cold-Arid Agricultural Sciences 2018 (11) 29-31

转录因子DREB1A基因的克隆及植物表达载体的构建

作  者:
柳娜;杨文雄
单  位:
甘肃省农业科学院小麦研究所
关键词:
转录因子;DREB1A基因;植物表达载体
摘  要:
研究转录因子DREB1A在植物抗渗透胁迫反应中的作用。根据GenBank中登录的DREB1A基因的序列设计引物,用PCR方法从拟南芥中克隆DREB1A基因,利用基因重组技术成功构建了植物表达载体pCAMB1A1300-DREB1A。该结果为进一步利用DREB1A基因做遗传转化研究奠定了基础。
译  名:
Cloning of Transcription Factor DREB1A Gene and Construction of Plant Expression Vector
作  者:
LIU Na;YANG Wenxiong;Institute of Wheat, Gansu Academy of Agricultural Sciences;
单  位:
LIU Na%YANG Wenxiong%Institute of Wheat, Gansu Academy of Agricultural Sciences
关键词:
Transcription factor;;DREB1A gene;;Plant expression vector
摘  要:
To research the function of the transcription factor DREB1 A in plant tolerance to osmotic stress, a pair of primers was designed according to the sequence of DREB1 A gene in GenBank databases. The DREB1 A gene was cloned from Arabidopsis thaliarra by the method of PCR. By the method of Recombinant DNA Technology, plant expression vector pCAMB1 A1300-DREB1 A was successfully constructed. The results laid a foundation for further research on genetic transformation using DREB1 A gene.

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