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当前位置: 首页 > 文章 > 低盐胁迫对三疣梭子蟹鳃和肝胰腺显微结构及家系存活的影响 渔业科学进展 2014,35 (1) 104-110
Position: Home > Articles > Effects of low salinity stress on microstructure of gill and hepatopancreas and family survival rate of Portunus trituberculatus Progress in Fishery Sciences 2014,35 (1) 104-110

低盐胁迫对三疣梭子蟹鳃和肝胰腺显微结构及家系存活的影响

作  者：
韩晓琳;高保全;王好锋;刘萍;陈萍;李华
单  位：
大连海洋大学;农业部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所
关键词：
三疣梭子蟹;盐度;存活率;鳃;肝胰腺;显微结构
摘  要：
在实验生态条件下研究了低盐环境对三疣梭子蟹鳃丝和肝胰腺显微结构、家系存活率的影响。研究结果表明,低盐(15.0、13.0、11.0、9.0)胁迫能够诱导鳃丝和肝胰腺显微结构改变。盐度为15.0时,上皮层变薄,B细胞数量增多;盐度为13.0时,鳃丝不规则增厚,B细胞数量进一步增多,肝小管中的R细胞的数量减少,柱状上皮细胞的细胞质内出现许多空泡;盐度为11.0时,上皮层出现解体,鳃腔中的血细胞明显增多,肝细胞空泡化现象更为严重;盐度为9.0时,上皮层破坏直至解体,B细胞中转运泡数量增多,体积增大,细胞结构损伤严重。以72h的低盐半致死盐度(LD50=11.1)为评价指标,检测24、48、72h共计10个家系对低盐的耐受性。各家系对照组(盐度为35.0)在实验期间均未出现死亡现象,而在低盐胁迫条件下,随着时间的逐渐延长,各家系的存活率均呈下降趋势,24、48、72h的存活率变化范围分别在64.44%~80.00%、50.00%~68.89%和33.33%~60.00%之间。各家系72h时的存活率大小顺序为J10>J4>J9>J5>J7>J3>J6>J2>J8>J1,均显著低于各自的对照组(P<0.05)。
译  名：
Effects of low salinity stress on microstructure of gill and hepatopancreas and family survival rate of Portunus trituberculatus
作  者：
HAN Xiao-lin;GAO Bao-quan;WANG Hao-feng;LIU Ping;CHEN Ping;LI Hua;Dalian Ocean University;Key Laboratory of Sustainable Development of Marine Fisheries,Ministry of Agriculture,Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences;
关键词：
Portunus trituberculatus;;Salinity;;Survival rate;;Gill;;Hepatopancreas;;Microstructure
摘  要：
The effects of low salinity on the microstructure of gill and hepatopancreas and family survival rate of Portunus trituberculatus were studied.The results showed that low salinity(15.0,13.0,11.0and 9.0)stress induced changes in microstructure of gill and hepatopancreas.At salinity of 15.0,the epithelium thinned slightly,and more B cells appeared in hepatopancreas tubules;At salinity of 13.0,the gill filament thickened irregularly,the number of B cells increased while R cells decreased,and many vacuoles appeared;At salinity of 11.0, disintegration of the epithelium was observed,the amount of haemocytes cells increased in gill cavity,and vacuoles in cytoplasm of columnar cells increased;At salinity of 9.0,epitheliumwas destroyed,even disassembled seriously,the number of vesicle shuttle in B cells increased and their volume enlarged,and the structure of hepatocytes was damaged seriously.Using the semi-lethal dose at 72hunder low salinity(LD50=11.1)as the evaluation index,low salinity tolerance of ten families at 24h,48hand 72hwas determined.No mortality was observed in the control during the experiment period.But the survival rate of the ten families decreased over time under low salinity stress,which was 64.44%-80.00%,50.00%-68.89%,and 33.33%-60.00%at 24h,48hand 72h,respectively.The ranking of survival rate of the ten families at 72hwas J10>J4>J9>J5>J7>J3>J6>J2>J8>J1,which significantly lower than the respective controls(P<0.05).

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