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Position: Home > Articles > Molecular Detection of Phytoplama of Jujube Witches Broom in Ningxia Autonomous Region Northern Horticulture 2010 (11) 166-168

宁夏枣疯病植原体分子检测

作  者:
魏天军;吴云峰;李百云
单  位:
宁夏农林科学院;西北农林科技大学
关键词:
枣;枣疯病;植原体;分子检测;宁夏回族自治区
摘  要:
运用国际上通用的植原体16S rRNA基因分子检测技术,对原产宁夏灵武市的灵武‘长枣品种’、‘灵武长枣1号’、‘灵武长枣2号’、‘灵武长枣3号’、‘灵武长枣4号’、‘灵武长枣5号’;原产宁夏中卫市的‘中卫大枣’和‘同心圆枣’和引进品种‘金昌1号’、‘敦煌大枣’(‘哈密大枣’)等10个品种品系样品的总DNA进行了巢式PCR扩增,结果这些材料没有携带枣疯病植原体,可以为飞速发展的宁夏枣产业繁育无毒优良品种苗木提供科技支撑。
译  名:
Molecular Detection of Phytoplama of Jujube Witches Broom in Ningxia Autonomous Region
作  者:
WEI Tian-jun1,WU Yun-feng2,LI Bai-yun1(1.Institute of Germplasm Resource,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan,Ningxia 750002;2.College of plant protection,Northwest Agriculture and Forestry University,Yangling,Shannxi 712100)
关键词:
Jjujube;jujube witchs'broom;phytoplasma;molecular detection;Ningxia Autonomous Region
摘  要:
Use of international common phytoplasma 16S rRNA gene molecule detection technology,the origin of Ningxia Lingwu city of Lingwuchangzao jujube varieties Lingwuchangzao No 1,Lingwuchangzao No 2,Lingwuchangzao No 3,Lingwuchangzao No 4,Lingwuchangzao No 5;origin Zhongwei in Ningxia Zhongwei City jujube varieties 'Tongxinyuanzao','Zhongweidazao' and introduced varieties 'Jinchang No 1','Dunhuangdazao'(Jujube Hami) in 10 samples of species,the total DNA was amplified by nested PCR.The results showed that these materials do not carry phytoplasma of jujube witches broom,the rapid development of Ningxia Jujube seedlings nursery non-toxic varieties provide a scientific and technological support.

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