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Position: Home > Articles > Combination of Matrix Solid Phase Dispersion Extraction with High Performance Liquid Chromatography for the Determination of Iprodione Residue in Fruits FOOD SCIENCE 2009,30 (14) 188-190

基质分散固相萃取-高效液相色谱法测定水果中的异菌脲残留量

作  者:
吴春红;聂继云;李静;李海飞;徐国锋
单  位:
中国农业科学院果树研究所
关键词:
分散固相萃取;液相色谱法;异菌脲;水果
摘  要:
以PSA(乙二胺基-N-丙基硅烷)和ODS(十八烷基硅胶键合硅胶填料)为混合固相基质分散剂萃取净化,液相色谱柱分离,建立了水果中异菌脲残留量的测定方法。异菌脲在0.1~5.0μg/ml的浓度范围内,峰面积和浓度的相关系数为0.9994。在香蕉、柑橘和芒果中分别添加0.5、1.0、2.0mg/kg三个浓度异菌脲,回收率在82.70%~108.62%之间,RSD值在1.8%~5.3%之间,方法的检出限为0.036mg/kg。此方法具有灵敏、准确、简便、重现性好等优点。
译  名:
Combination of Matrix Solid Phase Dispersion Extraction with High Performance Liquid Chromatography for the Determination of Iprodione Residue in Fruits
作  者:
WU Chun-hong,NIE Ji-yun*,LI Jing,LI Hai-fei,XU Guo-feng (Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China)
关键词:
matrix solid phase dispersion extraction;liquid chromatography;iprodione;fruits
摘  要:
A high performance liquid chromatographic method was developed for the determination of iprodione in fruits. Samples were extracted using acetonitrile, cleaned up by solid phase extraction using PSA and ODS (1:1, m/m) as dispersants. The chromatographic seperation was achieved on an ODS reverse-phase (RP) column. There was a good linear calibration curve in the range from 0.1 to 5.0 μg/ml with the correlation coefficient of 0.9994. The recoveries for iprodione in banana, orange and mango spiked at 0.5, 1.0 and 2.0 mg/kg ranged from 82.70% to 108.62%, with the RSDs of 1.8%-5.3%. The method detection limit was 0.036 mg/kg. This method is simple, sensitive, accurate and reproducible.

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