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柴芍口服液中芍药苷含量的高效液相色谱法测定

作  者:
张会梅;孙亚磊;王艳玲;钟英杰;魏秀丽;付海宁;栾明娜;庞云露;蒋贻海;徐恩民
单  位:
青岛蔚蓝生物股份有限公司
关键词:
柴芍口服液;高效液相色谱;芍药苷
摘  要:
采用高效液相法对柴芍口服液指标性成分芍药苷含量进行测定.色谱柱:Zorbax SB C18(4.6 mm*250 mm,5μm);流动相:乙腈+0.05%磷酸溶液(14∶86);流速:1.0 mL/min;柱温:35℃;检测波长:230 nm.经精密度、稳定性、重复性、回收率等验证,在该色谱条件下能够达到对柴芍口服液的质量控制.
作  者:
Zhang Huimei;Sun Yalei;Wang Yanling;Zhong Yingjie;Wei Xiuli;Fu Haining;Luan Mingna;Pang Yunlu;Jiang Yihai;Xu Enmin;Qingdao Vland Biotech INC Co., Ltd.;Qingdao National Engineering Technology Research Center of Animal Health Care Products Co., Ltd.;Shandong Veterinary Drug Quality Inspection Institute;
单  位:
Zhang Huimei%Sun Yalei%Wang Yanling%Zhong Yingjie%Wei Xiuli%Fu Haining%Luan Mingna%Pang Yunlu%Jiang Yihai%Xu Enmin%Qingdao Vland Biotech INC Co., Ltd.%Qingdao National Engineering Technology Research Center of Animal Health Care Products Co., Ltd.%Shandong Veterinary Drug Quality Inspection Institute
关键词:
Chaishao Oral Liquid;;High performance liquid chromatography(HPLC);;Paeoniflorin
摘  要:
The content of paeoniflorin in Chaishao Oral Liquid was determined by high performance liquid chromatography. The column was Zorbax SB C18(4.6 mm × 250 mm, 5 μm), the mobile phase was acetonitrile+0.05% phosphoric acid solution(14∶86), the flow rate was 1.0 mL/min, the column temperature was 35℃,and the detection wavelength was 230 nm.Under these conditions,the quality control of Chaishao Oral Liquid could be achieved by the verification of precision,stability,repeatability and rate of recovery.

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