中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 底物和产物及其类似物对植酸酶的抑制动力学 山东农业大学学报(自然科学版) 2005,36 (2) 235-240
Position: Home > Articles > INHIBITION KINETICS OF SUBSTRATE, PRODUCT AND ITS ANALOGUES ON THE PHYTASE Journal of Shandong Agricultural University(Natural Science Edition) 2005,36 (2) 235-240

底物和产物及其类似物对植酸酶的抑制动力学

作  者：
宋耿云;王敏;王晓云
单  位：
山东农业大学生命科学学院
关键词：
植酸酶;底物;产物;抑制动力学
摘  要：
本文研究了底物(植酸)、产物类似物(钒酸盐)和产物(磷酸盐)对植酸酶的活力影响。研究发现当植酸的浓度小于0.6mmol/L时,反应速度随着底物浓度的增加而增大;当植酸的浓度在0.6～1.6mmol/L范围内时,反应速度变化不大,维持一个平台期;而当浓度大于1.6mmol/L时,反应速度随着底物浓度的增加而降低,高浓度植酸对植酸酶有明显的抑制作用,动力学分析表明底物抑制常数K′s为2.0mmol/L。分别以植酸和对硝基苯磷酸二钠(pNPP)做底物时研究了钒酸盐对植酸酶的抑制作用,均表现为非竞争性抑制,抑制常数Ki分别为68.29μmol/L和2.42μmol/L。磷酸盐对植酸酶表现为竞争性抑制类型,抑制常数Ki值为1.45mmol/L。
译  名：
INHIBITION KINETICS OF SUBSTRATE, PRODUCT AND ITS ANALOGUES ON THE PHYTASE
作  者：
SONG Geng-yun, WANG Min, WANG Xiao-yun* (College of Life Science, Shandong Agriculture University, Taian, 271018,China)
关键词：
phytase, substrate, product, inhibition.
摘  要：
This paper studied the effects of a substrate, a product (phosphate) and its analog (vanadate) on the phytase activity. When the concentration of phytate was less than 0.6 mmol/L, the reaction rate increased with the increasing concentration of phytate. And when it was between 0.6 mmol/L and 1.6 mmol/L, the reaction rate changed little, but when it was more than 1.6 mmol/L, the reaction rate decreased with the increasing concentration of phytate. The result showed that high concentrations of phytate inhibited the phytase activity. The K's value for phytate was estimated to be 2.0 mmol/L. The enzyme activity was inhibited by vanadate with phytate and pNPP as substrate, respectively. The inhibition type was uncompetitive, with the Ki values 62.89 μmol/L and 2.42 μmol/L, respectively. The enzyme activity was also inhibited by phosphate with pNPP as substrate and the inhibition type was competitive with the Ki 1.45 mmol/L.

相似文章

导出引用
计量
文章访问数: 17
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46