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当前位置: 首页 > 文章 > 4个滇牡丹天然居群遗传多样性的ISSR分析 西部林业科学 2014 (3) 31-36
Position: Home > Articles > ISSR Analysis of Genetic Diversity among 4 Natural Populations of Paeonia delavayi Journal of West China Forestry Science 2014 (3) 31-36

4个滇牡丹天然居群遗传多样性的ISSR分析

作  者：
刘通;冯丹;陈少瑜;赵一鹤
单  位：
云南省林业科学院;西南林业大学
关键词：
滇牡丹;居群;遗传多样性;ISSR分子标记
摘  要：
以云南省香格里拉县4个滇牡丹天然居群为研究对象,采用ISSR分子标记技术,对98份材料进行遗传多样性分析,用多态性高、稳定性强的10条引物进行扩增,共获得96条扩增产物,其中多态性条带有82条,多态性百分比为85.42%;滇牡丹总体基因多样性为0.343 8,Shannon指数为0.500 9;通过Nei’s和聚类分析,居群间的遗传分化系数为0.797 3,居群间的遗传变异占总遗传变异的79.73%,而居群内的遗传变异只有20.27%,表明滇牡丹居群间的遗传分化较大,遗传变异主要存在于居群间。因此,滇牡丹虽然为分布区狭窄的特有种,但是与一些典型稀有和濒危的物种相比,遗传多样性并不低,滇牡丹的分布区域和种群数量呈现狭窄化、缩小化的趋势,主要原因是受人为过度采挖,生境受到破坏所致。
译  名：
ISSR Analysis of Genetic Diversity among 4 Natural Populations of Paeonia delavayi
作  者：
LIU Tong;FENG Dan;CHEN Shao-yu;ZHAO Yi-he;Environmental Science and Engineering College of Southwest Forestry University;Yannan Academy of Forestry;
关键词：
Paeonia delavayi;;population;;genetic diversity;;ISSR molecular marker
摘  要：
ISSR molecular marker technology was used to analyze genetic diversity of 4 natural populations of 98Paeonia delavayi individuals from shangri-la in Yunnan. 10 primers with high polymorphism and steady expansion were selected and 96 bands were identified,of which 82 bands were polymorphic,the polymorphism percentage was 85. 42 %. The total genetic diversity of 98 Paeonia delavayi was 0. 343 8,and the Shannon index was0. 500 9. Through the Nei's and Cluster analysis,the gene differentiation coefficient among populations was0. 797 3,the genetic variation among populations was 79. 73 %,the genetic variation within populations was only20. 27 %. All of these indicates that the different populations of Paeonia delavayi had a larger genetic differentiation,and the main genetic variation was existed among populations. The study also showed that although Paeonia delavayi was an endemic species,compared with some typical rare and endangered species,its genetic diversity was not low. The reason of its narrowing distribution tendency was mainly caused by over collection and habitat disturbance from human activities.

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