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Position: Home > Articles > Establishment of MITE Amplification System in the Wheat Study Acta Agriculturae Boreali-Sinica 2014,29 (2) 110-115

小麦MITE扩增体系的建立

作  者:
谢欢;杨文香;彭巧慧;赵丽娟;刘力伟;张娜;刘大群
单  位:
河北农业大学;邯郸市种子管理站;河北省农林科学院
关键词:
小麦叶锈病;抗病基因;MITE;分子标记
摘  要:
以Thatcher及23个以Thatcher为遗传背景的小麦抗叶锈病近等基因系为材料,开展了MITE扩增体系的建立研究,获得了适合小麦MITE分子标记的扩增体系及不同引物的退火温度,所用56对引物有27对获得稳定扩增。
译  名:
Establishment of MITE Amplification System in the Wheat Study
作  者:
XIE Huan;YANG Wen-xiang;PENG Qiao-hui;ZHAO Li-juan;LIU Li-wei;ZHANG Na;LIU Da-qun;College of Plant Protection,Agricultural University of Hebei,Biological Control Center of Plant Diseases and Plant Pests of Hebei Province,National Engineering Research Center for Agriculture in Northern Mountainous Areas;Hebei Academy of Agriculture and Foresty Sciences;Plant Seeds Management Station of Handan;
关键词:
Wheat leaf rust;;Resistance gene;;MITE;;Molecular markers
摘  要:
Thatcher and 23 of wheat leaf rust resistance near isogenic lines in Thatcher background were chosen as materials in this experiment. Research on the establishment of MITE amplification system was carried out. 27 of the 56 primer pairs were amplified stably,reaction system and different annealing temperature of primers were confirmed for amplification of MITE( Miniature inverted repeat transposable elements).

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