Dynamic distribution of HP-PRRSV JXA1 isolate in Tibetan,ZangMei black and Yorkshire pigs after infection tested by real-time quantitative PCR

KANG Rumin;JI Gaosheng;ZHOU Long;ZENG Fanya;WANG Hongning;Key Laboratory of Animal Disease Prevention and Food Safety of Sichuan Province,School of Life Sciences,Sichuan University;Key Laboratory of Animal Breeding and Genetics Sichuan Province,Sichuan Academy of Animal Sciences;

JXA1 isolate;;Tibetan pig;;ZangMei black pig;;Yorkshire pig;;real-time quantitative PCR;;dynamic distribution

In order to investigate viral distribution in PRRSV-infected Tibetan,Zang Mei black and Yorkshire pigs,we used 1 m L 4×105 TCID50/ml of HP-PRRSV isolate to infect Tibetan,Zang Mei black and Yorkshire pigs( 15 for each breed) and measured viral RNA loads in each of the fifteen tested organs of the pigs on 0,4,7,14 days after PRRSV challenge. The 15 tested organs were heart,liver,spleen,lung,kidney,brain,small intestine,mesenteric lymph node,groin lymph node,submandibular gland,mandibular lymph node,thyroid,thymus,bronchial lymph nodes and tonsil. The results were that viral RNA was identified in all the fifteen organs of Zang Mei black and Yorkshire pigs,but not in the heart,liver,spleen,kidney,small intestine,submandibular gland and thyroid of the Tibetan pigs during infection.The maximum viral RNA loads in the organs of the three pig breeds 4,7,and 14 days after infection were evidently different,with the highest loads in the amygdala,inguinal lymph nodes and lymph nodes of lower jaw of the Tibetan pigs,in the spleen and lung of Zang Mei black pigs,and in the thymus,lung and lower jaw lymph of the Yorkshire pigs. To sum up,HP-PRRSV infection results in different distribution patterns of virus in the three pig breeds as a whole and in different organs of individual breeds of the pigs.