Position: Home > Articles > Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV
China Animal Husbandry & Veterinary Medicine
2017,44
(2)
538-545
不同品种猪感染HP-PRRSV后肺组织间PRRSV受体基因差异表达的研究
作 者：
康润敏;姬高升;曾凯;吕学斌;殷明郁
单 位：
四川省畜牧科学研究院;四川大学生命科学学院
关键词：
JXA1分离株;藏猪;藏梅猪;大约克夏猪;PRRSV受体基因;差异表达
摘 要：
为进一步了解藏猪、藏梅猪和大约克夏猪对高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)JXA1分离株具有不同易感性的分子机理,本研究建立实时荧光定量RT-PCR方法比较分析藏猪、藏梅猪和大约克夏猪感染JXA1分离株前、感染后4、7和14d肺脏中HSPG2、SIGLEC1、CD163、VIM和NMMHC-ⅡA5个PRRSV受体基因的差异表达情况。结果显示,藏猪感染JXA1分离株后4和14d肺脏中HSPG2的表达量显著高于感染前(P<0.05),而感染后7dHSPG2的表达量显著低于感染前(P<0.05),藏梅猪在感染后14dHSPG2的表达量显著高于感染后7d(P<0.05);藏猪感染后4和14d肺脏中SIGLEC1的表达量显著高于感染前(P<0.05),大约克夏猪感染后4、7和14dSIGLEC1的表达量均显著低于感染前(P<0.05);藏猪和藏梅猪感染后14d肺脏中CD163的表达量均显著高于感染前(P<0.05),大约克夏猪则感染后7和14d均显著低于感染前(P<0.05);藏猪感染后7d肺脏中VIM的表达量显著高于感染前(P<0.05),大约克夏猪在感染后7d显著低于感染前(P<0.05);藏梅猪感染后4d肺脏中NMMHC-ⅡA的表达量显著高于感染前(P<0.05),大约克夏猪在感染后4和14dNMMHC-ⅡA的表达量显著高于感染前(P<0.05)。结果表明,5个PRRSV受体基因中,SIGLEC1和VIM基因可能是影响藏猪、藏梅猪和大约克夏猪对JXA1分离株易感性存在差异的潜在关键基因。
译 名：
Study on Differential Expression of PRRSV Receptor Genes in Lung Tissues of Different Breeds of Pig Infected with HP-PRRSV
作 者：
KANG Run-min;JI Gao-sheng;ZENG Kai;LV Xue-bin;YIN Ming-yu;Animal Breeding and Genetics Key Laboratory of Sichuan Province,Sichuan Animal Science Academy;Key Laboratory of Bio-resources and Ecoenvironment of Ministry of Education,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,College of Life Sciences,Sichuan University;
关键词：
JXA1isolate;;Tibetan pig;;ZangMei Black;;Yorkshire;;PRRSV receptor genes;;differential expression
摘 要：
In order to understand the molecular mechanism of host susceptibility differential to highly pathogenic porcine reproductive and respiratory syndrome(HP-PRRSV)infection among three breeds of pig,Tibetan pig,ZangMei Black and Yorkshire,the mRNA level of five reported PRRSV receptor genes(HSPG2,SIGLEC1,CD163,VIM and NMMHC-ⅡA)expressed in lung were investigated on 0,4,7and 14 days post infection(dpi)with HP-PRRSV JXA1 isolate.The Real-time RT-PCR results showed that the expression of HSPG2 in Tibetan pig increased significantly on 4and 14 dpi and decreased significantly on 7dpi compared with the expression on 0dpi(P<0.05),and its expression in ZangMei Black on 14 dpi was significantly higher than that of on 7dpi(P<0.05);An increased expression of SIGLEC1 in Tibetan pig was observed on 4and14dpi(P<0.05),and a decreased expression of the same gene was detected in Yorkshire on 4,7and 14dpi(P<0.05);The expression of CD163 elevated significantly on 14 dpi in both Tibetan pig and ZangMei Black(P<0.05),however its expression reduced significantly on 7and 14 dpi in Yorkshire(P<0.05);The expression of VIM gene was significantly higher on 7dpi in Tibetan pig and significantly lower on 7dpi in Yorkshire(P<0.05);And the expression of NMMHC-ⅡA was significantly higher on 4dpi in ZangMei Black,which was also significantly higher in Yorkshire on 4and 14dpi(P<0.05).In conclusion,SIGLEC1 and VIM might be important genes to influence the host resistance of Tibetan pig,ZangMei Black and Yorkshire to HP-PRRSV infection.
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