当前位置: 首页 > 文章 > 辣椒根腐病内生拮抗细菌Hj33-7发酵条件研究 沈阳农业大学学报 2010,41 (6) 677-680
Position: Home > Articles > Fermentation Conditions of Endophytic Antagonistic Bacteria Hj33-7 Isolated from Pepper with Antifungal Activity Against Fusarium oxysporum Journal of Shenyang Agricultural University 2010,41 (6) 677-680

辣椒根腐病内生拮抗细菌Hj33-7发酵条件研究

作  者:
祁之秋;李修伟;李兴海;王英姿;张杨;纪明山
单  位:
沈阳农业大学植物保护学院
关键词:
辣椒根腐病;抗菌细菌;发酵条件;抑菌活性
摘  要:
为提高辣椒根腐病内生拮抗细菌Hj33-7发酵生产的抗菌活性,对其摇床发酵培养基成分及发酵温度、pH值、通气量、接种量和发酵时间5个发酵条件进行研究。结果表明:在1000mL含麦芽糖20g,酵母浸膏5g,蛋白胨10g的液体培养基中最有利于菌体产生和抗菌活性的提高;最适宜发酵条件为发酵温度28℃,pH值为7,在250mL三角瓶的装液量为20mL,接种量30%,130r.min-1发酵培养48h菌体产生量最大,抗菌活性最高。
译  名:
Fermentation Conditions of Endophytic Antagonistic Bacteria Hj33-7 Isolated from Pepper with Antifungal Activity Against Fusarium oxysporum
作  者:
QI Zhi-qiu,LI Xiu-wei,LI Xing-hai,WANG Ying-zi,ZHANG Yang,JI Ming-shan(College of Plant Protection,Shenyang Agricultural University,Shenyang 110866,China)
关键词:
Fusarium oxysporum;antagonistic bacteria;fermentation condition;antifungal activity
摘  要:
The compositions of cultural medium and the fermentation conditions of endophytic antagonistic bacteria Hj33-7 strain isolated from pepper with antifungal activity were studied by using the orthogonal design method.The results showed that the fermentation medium was composed of maltone 2.0%,yeast 0.5% and peptone 1% in favor of growth and antimicrobial activity of strain Hj33-7.The optimal fermentation parameters were 28℃,pH 7.0,20mL culture in 250mL flask,30% inoculation volume and 130 r·min-1 for 48h,which had the strongest growth and antagonistic activity of Hj33-7 strain.

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