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Position: Home > Articles > Research Progress on Genes Related to Steviol Glycosides Biosynthesis in Stevia Sugar Crops of China 2019,41 (3) 70-76

甜菊糖生物合成相关基因的研究进展

作  者:
倪洪涛;董花;周艳丽
单  位:
甘肃省武威市凉州区农产品质量安全监督管理站;黑龙江大学农业资源与环境学院
关键词:
甜叶菊;甜菊糖;生物合成;基因;UDP-葡糖基转移酶
摘  要:
甜菊糖(Steviol glycosides,SGs)中商用的蛇菊苷(Stevioside,STV)和莱鲍迪苷A(Rebaudioside A,Reb A),是经甲基赤藓糖醇(MEP)途径,由最初的物质丙酮酸和甘油醛-3-磷酸在各种酶的催化作用下,一步步先合成牻牛儿牻牛儿焦磷酸(GGPP),然后GGPP在几种酶基因作用下转化为甜菊醇(Steviol),之后在UDP-葡糖基转移酶(UGTs)基因的作用下形成STV和Reb A,整个过程共有15个酶基因参与.本文综述了甜叶菊中参与甜菊糖生物合成过程UDP-葡糖基转移酶等基因及其它基因的克隆、表达、调节等作用及不同外界环境条件下基因表达差异的研究进展.搞清这些酶基因的作用机理,可有意地、更好地调控甜菊糖的生产量和品质,为改善甜菊糖的生产提供参考.
译  名:
Research Progress on Genes Related to Steviol Glycosides Biosynthesis in Stevia
作  者:
Ni Hongtao;Dong Hua;Zhou Yanli;College of Agricultural Resources and Environment, Heilongjiang University;Quality and Safety Supervision and Management Station of Agricultural Products of Liangzhou District;
关键词:
stevia;;steviol glycosides;;biosynthesis;;genes;;UDP-glucosyltransferases
摘  要:
The commercial stevioside(STV) and rebaudioside A(Reb A) in stevioside(steviol glycosides, SGs) are in the pathway of 2-C-methyl-D-erythritol-4 phosphate(MEP), the initial substances pyruvate and glyceraldehyde-3-phosphate catalyzed by various enzymes, step by step synthesis of geranyl-geranyl diphosphate(GGPP), then GGPP is transformed into steviol under the action of several enzyme genes, and steviol transformed into STV and Reb A under UDP-glucosyltransferase(UGTs) gene, the whole process involved 15 enzyme genes. In this paper, the research progress of genes cloning, expression and regulation such as UDP-glucosyltransferase and other genes involved in stevia biosynthesis were reviewed. Understanding the mechanism of these enzyme genes can regulate the production and quality of steviol glycosides, and provide reference for improving the production of steviol glycosides.

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