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Position: Home > Articles > Inhibition of tea extracts on glucose uptaking by polarized intestinal Caco-2 cells Journal of Huazhong Agricultural University 2013,32 (1) 116-119

茶叶提取物对小肠Caco-2细胞糖吸收的抑制作用

作  者:
倪德江;陈玉琼;余志;彼得 R.埃利斯;克里斯托弗 P.科尔佩
单  位:
华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室;School
关键词:
茶叶提取物;Caco-2细胞;葡萄糖吸收;儿茶素;糖尿病
摘  要:
利用水提法制备绿茶、乌龙茶、红茶、青砖茶和普洱茶提取物,研究5种提取物对体外小肠Caco-2细胞吸收葡萄糖的抑制效果。结果表明:在相同质量浓度(0.5mg/mL)条件下,5种茶叶提取物均能抑制Caco-2细胞吸收葡萄糖的活性,抑制能力大小依次为绿茶!乌龙茶!红茶!青砖茶!普洱茶;与对照相比,绿茶、乌龙茶、红茶和青砖茶提取物极显著抑制葡萄糖的吸收,但普洱茶提取物的抑制效果并未达到显著水平(P>0.05)。茶叶提取物抑制小肠Caco-2细胞吸收葡萄糖的能力可能与加工过程中儿茶素的转化有关。
译  名:
Inhibition of tea extracts on glucose uptaking by polarized intestinal Caco-2 cells
作  者:
NI De-jiang1 CHEN Yu-qiong1 YU Zhi1 Peter R.Ellis2 Christopher P.Corpe2 1.College of Horticulture and Forestry Sciences/Key Laboratory of Horticultural Plant Biology, Ministry of Education,Huazhong Agricultural University,Wuhan 430070,China; 2.Biopolymers,School of Biomedical and Health Sciences, King's College London,SE1 9NH London,United Kingdom
关键词:
tea extract;Caco-2 cell;glucose transport;catechin;diabetes
摘  要:
The green tea extract(GT),oolong tea extract(OT),black tea extract(BT),Qingzhuan tea extract(QT) and Pu-erh tea extract(PT) were prepared by using water and the effect of them on intestinal glucose uptaking was investigated using polarized Caco-2 intestinal cells.The extracts(0.5 mg/mL) inhibited glucose uptaking into the intestinal Caco-2 cells under sodium-dependent conditions in the order:GT>OT>BT>QT>PT.Compared with control,GT,OT,BT and QT inhibited significantly glucose-uptaking(P<0.01),except for PT(P>0.05).Catechin may be the predominant inhibitor in tea and its conversion during manufacturing could affect the glucose-uptake inhibitory activity.

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