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当前位置: 首页 > 文章 > 新型消毒剂过碳酸钠灭菌对烟草种子萌发和无菌苗生长的影响 中国农学通报 2018 (10) 43-47
Position: Home > Articles > Effect of Sterilization with Sodium Percarbonate on Seed Germination and Plant Growth of Tobacco Chinese Agricultural Science Bulletin 2018 (10) 43-47

新型消毒剂过碳酸钠灭菌对烟草种子萌发和无菌苗生长的影响

作  者：
王一珂;陶向;马欣荣
单  位：
中国科学院成都生物研究所;成都市第七中学
关键词：
过碳酸钠;次氯酸钠;升汞;烟草种子;表面灭菌;萌发;植株生长
摘  要：
为探索和评价新型消毒剂在植物表面灭菌中的应用方法,本研究比较过碳酸钠、次氯酸钠、升汞3种灭菌剂对烟草种子的表面灭菌效果,及对种子萌发和植株生长的影响。烟草种子分别用4.0%过碳酸钠水溶液(活性氧含量≥0.54%)处理20~40 min后直接接种于MS培养基中;33.3%次氯酸钠溶液(活性氯含量≥1.65%)处理10 min或0.1%升汞水溶液灭菌4 min,无菌水清洗5次后接种于MS培养基中。3种方法灭菌效果相当,无污染,并且90%的种子能萌发。过碳酸钠和次氯酸钠处理后种子萌发快、植株生长良好,且过碳酸钠灭菌组的植株生长略优;而升汞则延缓种子萌发并抑制植株生长。此外,因过碳酸钠灭菌后无需清洗,显示了其作为种子表面灭菌剂的极大优势。本研究建立了烟草种子表面灭菌的新方法,同时为其他植物的种子及外植体表面灭菌提供了参考,具有较大的实用价值。
译  名：
Effect of Sterilization with Sodium Percarbonate on Seed Germination and Plant Growth of Tobacco
作  者：
Wang Yike;Tao Xiang;Ma Xinrong;Chengdu No.7 High School;Chengdu Institute of Biology, Chinese Academy of Sciences;
关键词：
sodium percarbonate;;sodium hypochlorite;;mercury chloride;;tobacco seed;;surface sterilize;;germination;;plant growth
摘  要：
Sodium percarbonate, a new disinfectant, has been widely used in the sterilization and cleaning, butis not reported in the surface sterilization of plant seeds and explants. In this study, tobacco seeds were used tostudy the effects of three sterilizing agents, including sodium percarbonate, sodium hypochlorite and mercuricchloride, on the seed surface sterilization and their effects on seed germination and plant growth. Tobaccoseeds were treated with 4.0% aqueous solution of sodium percarbonate(active oxygen content ≥0.54%) for 20-40 min and then inoculated directly onto MS medium for germination and growth. While the other two groupswere sterilized separately with 33.3% sodium hypochlorite solution(active chlorine content ≥1.65%) for 10 minand 0.1% aqueous solution of mercury for 4 min, then cleaned for 5 times with sterile water and inoculated onthe same medium. The results showed that 90% of seeds germinated and no plantlet was contaminated,indicating that the three methods were effective. The seeds germinated fast and the plantlets grew well insodium percarbonate and sodium hypochlorite treatments, and the group of sodium percarbonate treatment wasslightly better than that of sodium hypochlorite. However, mercury delayed seed germination and inhibitedplant growth. In addition, sodium percarbonate eliminated the need for cleaning after sterilization, exhibiting agreat advantage. This study establishes a new method for tobacco seed surface sterilization, which provides a reference for the sterilization of other plants seeds and explants, showing huge practical value.

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