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当前位置: 首页 > 文章 > 低磷胁迫对太空诱变耐低磷柱花草酸性磷酸酶活性和磷效率的影响 草业学报 2018 (8) 78-85
Position: Home > Articles > Effects of phosphorus deficiency on acid phosphatase activity and phosphorus efficiency in a low-phosphorus tolerant Stylosanthes mutant Acta Prataculturae Sinica 2018 (8) 78-85

低磷胁迫对太空诱变耐低磷柱花草酸性磷酸酶活性和磷效率的影响

作  者：
刘攀道;郇恒福;刘一明;刘国道;白昌军;陈志坚
关键词：
柱花草;低磷胁迫;酸性磷酸酶;磷效率;太空诱变
摘  要：
低磷胁迫是酸性土壤限制牧草生产的主要障碍因子之一。本课题组前期的研究中,通过太空诱变获得了一个耐低磷柱花草突变体TPRC2001-84,但其适应低磷胁迫的机制仍不清楚。以TPRC2001-84及其亲本热研2号(RY2)为试验材料,通过水培方法,比较正常供磷(+P,250μmol·L-1 KH2PO4)和低磷(-P,5μmol·L-1KH2PO4)处理对TPRC2001-84和RY2生物量、磷吸收效率、磷利用效率、可溶性磷浓度、细胞壁磷含量和酸性磷酸酶(ACP)活性的影响。结果表明,相对于正常供磷处理,低磷处理显著降低了两份柱花草材料的植株干重和磷吸收效率(P<0.05),但显著提高了两份柱花草材料的磷利用效率(P<0.05)。并且,TPRC2001-84在低磷处理下的植株干重和磷利用效率分别是RY2的1.6和1.4倍。在低磷胁迫下,虽然TPRC2001-84叶片和根系的细胞壁磷含量只有RY2的70%左右,但是,TPRC2001-84叶片和根系的可溶性磷浓度分别比RY2高36.8%~42.6%,细胞壁ACP活性分别比RY2高46.6%~53.6%。以上结果说明,柱花草TPRC2001-84在低磷胁迫下具有较高的细胞壁ACP活性,有利于其对细胞壁中贮存磷的活化利用,从而获得较高的磷利用效率。这将为选育磷高效柱花草新品种提供理论依据和种质材料。
译  名：
Effects of phosphorus deficiency on acid phosphatase activity and phosphorus efficiency in a low-phosphorus tolerant Stylosanthes mutant
作  者：
LIU Pan-dao;HUAN Heng-fu;LIU Yi-ming;LIU Guo-dao;BAI Chang-jun;CHEN Zhi-jian;Institute of Tropical Crop Genetic Resources,Chinese Academy of Tropical Agriculture Sciences & Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture;
单  位：
LIU Pan-dao%HUAN Heng-fu%LIU Yi-ming%LIU Guo-dao%BAI Chang-jun%CHEN Zhi-jian%Institute of Tropical Crop Genetic Resources,Chinese Academy of Tropical Agriculture Sciences & Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China,Ministry of Agriculture
关键词：
Stylosanthes;;phosphorus deficiency;;acid phosphatase;;phosphorus efficiency;;space flight mutation
摘  要：
Low phosphorus(P)availability is a major constraint for pasture yields in acid soils.In a previous study,a low-P tolerant stylo(Stylosanthes)mutant,TPRC2001-84,was created using space flight mutation.However,the underlying mechanisms of TPRC2001-84 tolerance of P deficiency remain largely unknown.In this study,plant biomass,P acquisition efficiency,P utilization efficiency,soluble inorganic phosphate(Pi)concentration,cell wall P content and acid phosphatase(ACP)activity were investigated in hydroponically grown TPRC2001-84 and a non-mutant control(RY2)in normal P(+P,250μmol·L-1 KH2 PO4)or low P(-P,5μmol·L-1 KH2 PO4)solutions.Plant dry weight and P acquisition efficiency were significantly decreased(P<0.05)by P deficiency,while P utilization efficiency was increased(P<0.05)by low P in both accessions,compared to the normal P treatment.Plant dry weight and P utilization efficiency of TPRC2001-84 were 1.6 and 1.4 times those of RY2 under P deficiency,respectively.Although cell wall P content in leaves and roots of TPRC2001-84 were about 70%lower than in RY2 with low P treatment,the soluble Pi concentration in leaves and roots of TPRC2001-84 were 36.8% and 42.6% higher than in RY2 under P deficiency,respectively.Similarly,cell wall ACP activity in leaves and roots of TPRC2001-84 were 46.6%and 53.6% higher than in RY2 under P deficiency,respectively.These results suggest that increased cell wall ACP activity in TPRC2001-84 may contribute to cell wall P scavenging and recycling,increasing P utilization efficiency under P deficiency.Taken together,our results provide a theoretical basis and germplasm resource for selecting and breeding stylo varieties with high P efficiency.

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