作 者：
刘自逵;刘国华;戴荣四;刘伟;李芬;胡涛;刘毅
关键词：
猬迭宫绦虫;线粒体DNA;cox1基因;nad1基因;种系发育关系
摘 要：
本研究旨在阐明猬迭宫绦虫湖南分离株线粒体细胞色素c氧化酶第I亚基(cox1)基因部分序列(pcox1)和烟酰胺腺嘌呤二核苷酸(NADH)脱氢酶亚单位l基因(nad1)部分序列(pnad1)的遗传变异情况,并用pcox1和pnad1序列重构猬迭宫绦虫与其它绦虫的种群遗传关系。利用聚合酶链反应(PCR)扩增猬迭宫绦虫的pcox1和pnad1,应用ClustalX1.81程序对序列进行比对,再用Phylip3.67程序MP法和Mage4.0程序NJ法绘制种系发育树,并用Puzzle5.2程序构建最大似然树,同时利用DNAStar5.0中的Megalign程序进行同源性分析。结果显示所获得各样品株的pcox1和pnad1序列长度一致,分别为396和566bp,湖南分离株与已知猬迭宫绦虫位于同一分枝。由于猬迭宫绦虫pcox1和pnad1序列种内相对保守,种间差异较大,故均可作为种间遗传变异研究的标记,从而为猬迭宫绦虫的种群体遗传学研究和其相关疾病的诊断奠定基础。
译 名：
Sequence and Phylogenetic Analysis of Mitochondrial cox1 and nad1 Genes for Spirometra erinaceieuropaei Isolates from Hunan Province
作 者：
LIU Zi-kui1,2,LIU Guo-hua1,DAI Rong-si1,LIU Wei1,LI Fen1,HU Tao1,LIU Yi1(1.College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China;2.Hunan Engineering Technology Center of Veterinary Drug,Changsha 410128,China)
关键词：
Spirometra erinaceieuropaei;mitochondrial DNA;cox1 gene;nad1 gene;phylogenetic relationship
摘 要：
The objectives of the present study were to examine sequence variation in the mitochondrial cytochrome coxidase subunit 1(cox1)gene and NADH dehydrogenase subunit 1(nad1)gene among Spirometra erinaceieuropaei isolates from Hunan Province,and to reconstruct their phylogenetic relationship using cox1 and nad1 sequences.The partial cox1(pcox1)and nad1(pnad1)was amplified from each S.erinaceieuropaei sample,and pcox1 and pnad1 sequences were aligned using the ClustalX 1.81.MP and NJ trees of pcox1 and pnad1 were constructed using the software Phylip 3.67 version 4.0 and Mage version 4.0,and ML tree was also constructed using Puzzle version 5.2.Sequence homology analysis was performed using the Megalign program of the software DNAStar version 5.0.The results showed that the lengths of pcox1 and pnad1 sequences were 396 and 566 bp,respectively.The constructed phylogenetic tree revealed that the Hunan isolates and the S.erinaceieuropaei available in GenBank were clustered in the same clade.There is no significant variation in pcox1 sequences within S.erinaceieuropaei,while inter-species difference is obvious.It is concluded that pcox1 and pnad1 sequences can be used as genetic marker for population genetic studies of cestodes.The results of the present study provided foundation for further studies of population genetics of S.erinaceieuropaei,and for diagnosis of the resultant disease.
