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当前位置: 首页 > 文章 > rac-甲霜灵及R-甲霜灵对斑马鱼急性毒性和ATP酶活性的研究 农业环境科学学报 2008,27 (06) 2456-2460
Position: Home > Articles > Effects of Rac-metalaxyl and R-metalaxyl on Zebrafish(Danio rerio):Determination of Acute Toxicity and ATPase Activity Journal of Agro-Environment Science 2008,27 (06) 2456-2460

rac-甲霜灵及R-甲霜灵对斑马鱼急性毒性和ATP酶活性的研究

作  者：
姚琨;朱琳;陈中智;端正花;李燕
单  位：
南开大学环境科学与工程学院
关键词：
手性农药;甲霜灵;斑马鱼;Na+,K+-ATP酶;Ca2+-ATP酶;毒性评价
摘  要：
为探讨农药的"手性"特征对非靶生物的毒性,采用急性毒性试验和生化测定方法,研究了手性农药rac-甲霜灵及R-甲霜灵对模式生物斑马鱼的致死和亚致死(ATP酶活性)效应。rac-甲霜灵对斑马鱼的24h和96h的LC50分别为258.47、241.98mg·L-1,R-甲霜灵对斑马鱼的24h和96h的LC50分别为237.67、227.38mg·L-1,都属于低毒农药。rac-甲霜灵和R-甲霜灵对斑马鱼Ca2+-ATP酶的活性,在96h的测定时间范围内都只表现出诱导效应,但R-甲霜灵对其的诱导效应更为显著,诱导时间也较长;相同浓度下R-甲霜灵比rac-甲霜灵对Na+,K+-ATP酶的活性诱导时间长,并且在高浓度诱导效应开始的早。结果表明R-甲霜灵比rac-甲霜灵对斑马鱼的急性毒性稍大,但两者在亚致死效应上存在较为明显的差异,表明斑马鱼体内可能存在对映选择性。
译  名：
Effects of Rac-metalaxyl and R-metalaxyl on Zebrafish(Danio rerio):Determination of Acute Toxicity and ATPase Activity
作  者：
YAO Kun1, ZHU Lin1,2, CHEN Zhong-zhi1, DUAN Zheng-hua1, LI Yan1 (1. College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China;2. Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education, Nankai University, Tianjin, 300071, China)
关键词：
rac-metalaxyl; R-metalaxyl; zebrafish; Na+,K+-ATPase; Ca2+-ATPase; toxic assessment
摘  要：
Many studies indicated that enantiomers of chiral pesticides differed in biological activity,which meant single enantiomer pesticides might had different effects on non-target organisms relative to racemate. So far, few studies whether R-metalaxyl brings about the same environmental effects on aquatic organisms as rac-metalaxyl has been reported. In order to provide more information for aquatic risk about metalaxyl, 24 h and 96 h acute toxicity of rac-metalaxyl and R-metalaxyl to adult zebrafish(Danio rerio)were tested, and the variance of ATPase activity in zebrafish under sublethal concentrations was investigated. The results showed both of them belonged to low-toxic pesticides based on LC50 values(24 h and 96 LC50 for rac-metalaxyl were 258.47 mg·L-1 and 241.98 mg·L-1;for R-metalaxyl were 227.38 mg·L-1 and 237.67 mg·L-1),and had a little difference in actue toxicity to zebrafish between the two compounds. In the case of ATPase test,the induction of ATPase activity was observed during the 96 h-test, and no inhibition effects observed. Moreover, induction effect on Ca2+-ATPase for R-metalaxyl was more significant than rac-metalaxyl. For Na+, K+-ATPase,R-metalaxyl began a induction earlier in the highest concentration exposed(70 mg·L-1),and had a longer time in induction than rac-metalaxyl. In general, the results showed a little difference in acute toxicity to zebrafish and a pronounced difference in effects on ATPase activity in zebrafish between rac-metalaxyl and R-metalaxyl. It is a possible explanation that enantioselective action occurred in zebrafish.

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