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当前位置: 首页 > 文章 > EST-SSR标记在翦股颖上的通用性及其利用 园艺学报 2010,37 (3) 485-490
Position: Home > Articles > Transferability of EST-SSR Markers on Bentgrasses and Its Application Acta Horticulturae Sinica 2010,37 (3) 485-490

EST-SSR标记在翦股颖上的通用性及其利用

作  者：
赵岩;孔凡美;王涛;李斯深
单  位：
山东农业大学资源与环境学院;作物生物学国家重点实验室
关键词：
翦股颖;EST-SSR;通用性;遗传多样性
摘  要：
利用来自小麦、大麦、玉米、高粱、水稻的1058对EST-SSR在翦股颖上进行扩增,得到有效扩增引物620对(58.60%)。其中源于小麦、大麦、玉米、高粱、水稻的引物对数分别为313(61.49%)、30(63.83%)、101(51.53%)、11(35.48%)、165(60.00%)。进一步利用其中53个引物对,对8个翦股颖品种进行了遗传多样性分析。有51对引物显示了多态性,占引物数的94.34%;共检测到368个等位变异,平均每对引物有6.9个等位变异;多态性信息指数(PIC)为0.49～0.93,平均为0.78;品种间遗传相似系数为0.48～0.86,平均为0.72。聚类分析结果表明,在相似系数0.75处,8个品种可明确的分为两大类,第Ⅰ类为高地;第Ⅱ类又可分为两个亚类,Ⅱ-1亚类由阿尔法、潘克劳斯、回旋组成,Ⅱ-2亚类由普特、L-93、开拓、A4组成。
译  名：
Transferability of EST-SSR Markers on Bentgrasses and Its Application
作  者：
ZHAO Yan1,2,KONG Fan-mei1,2,WANG Tao 2,and LI Si-shen1,(1 State Key Laboratory of Crop Biology/College of Agronomy,Shandong Agricultural University,Tai'an,Shandong 271018,China;2 College of Resources and Environment,Shandong Agricultural University,Tai'an,Shandong 271018,China)
关键词：
bentgrass;EST-SSR;transferability;genetic diversity
摘  要：
Bentgrass is a major cool season turfgrass.Few molecular markers have been developed for bentgrass compared to field crops.In this study,1 058 EST-SSR primer pairs developed by our lab from wheat,barley,maize,sorghum and rice were screened on bentgrass.Of which,620(58.60%) primer pairs showed amplification.Effective primer pairs from wheat,barley,maize,sorghum and rice were 313(61.49%),30(63.83%),101(51.53%),11(35.48%),and 165(60.00%),respectively.Furthermore,53 EST-SSR primers were selected to investigate the genetic relationship of eight bentgrass cultivars,and 51(94.34%) primer pairs showed polymorphism.In total,368 alleles were found with an average of 2.69 alleles per primer.The PIC(polymorphism information content) for each polymorphic primer varied from 0.49 to 0.93,with an average of 0.78.The average genetic similarity coefficient(GS) among the eight cultivars was 0.72(ranged from 0.48 to 0.86).Clustering analysis indicated that the eight bentgrass cultivars were clustered into two groups based on 0.75 of GS.GroupⅠconsists only Highland,group Ⅱ includes the others,which could be divided into two subgroups based on 0.80 of GS.The sub-group Ⅱ-1 includes Alpha,Penncross and Blackspin,The sub-group Ⅱ-2 includes Putter,L-93,Cato and Penn A4.

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