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当前位置: 首页 > 文章 > 紫苏PfPDCT基因序列特征及表达分析 山西农业科学 2019 (5)
Position: Home > Articles > 紫苏PfPDCT基因序列特征及表达分析 Journal of Shanxi Agricultural Sciences 2019 (5)

紫苏PfPDCT基因序列特征及表达分析

作  者：
任文燕;周雅莉;杨慧娟;郝月茹;杨宏斌;李润植;王计平
单  位：
山西农业大学农学院
关键词：
紫苏;磷脂酰胆碱甘油二脂转磷酸胆碱酶(PDCT)基因;脂肪酸代谢;生物信息学分析;表达特性
摘  要：
采用生物信息学技术和在线分析软件对紫苏PfPDCT基因序列及所编码的蛋白质进行分析,并且利用实时荧光定量PCR技术研究该基因在晋紫苏1号种子发育不同时期的表达特性,旨在探究磷脂酰胆碱甘油二脂转磷酸胆碱酶(PDCT)在植物脂肪酸代谢过程的作用.结果表明,紫苏PfPDCT基因c DNA全长序列为2 098 bp,开放阅读框为1 683 bp,编码560个氨基酸残基.生物信息学分析结果表明,PfPDCT蛋白分子量约为59.315 ku,等电点为9.48,不稳定系数为35.00,推测其为稳定蛋白,与已知赤藓PDCT蛋白高度同源.通过荧光定量PCR分析可知,紫苏PfPDCT基因在不同发育时期的种子中均有表达,其中,在开花后20 d表达量最高,为开花后10 d的1.92倍.研究结果可为进一步阐明紫苏PfPDCT基因的功能及作用机制奠定基础.
作  者：
REN Wenyan;ZHOU Yali;YANG Huijuan;HAO Yueru;YANG Hongbin;LI Runzhi;WANG Jiping;College of Agronomy,Shanxi Agricultural University;Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University;
单  位：
REN Wenyan%ZHOU Yali%YANG Huijuan%HAO Yueru%YANG Hongbin%LI Runzhi%WANG Jiping%College of Agronomy,Shanxi Agricultural University%Institute of Molecular Agriculture and Bioenergy,Shanxi Agricultural University
关键词：
Perilla frutescens;;PfPDCT gene;;fatty acid metabolism;;bioinformatics analysis;;expression characteristics
摘  要：
This study was conducted to explore the role of PfPDCT enzyme in the process of the plant fatty acids metabolism. The PfPDCT gene and the encoded protein were analyzed using bioinformatics techniques and online software. And the PfPDCT gene expression characteristics in Perilla frutescens seeds from different development stages of Jinzisu 1 were analyzed. The results showed that the full-length c DNA sequence of PfPDCT gene was 2 098 bp, with a 1 683 bp ORF, which encoded 560 amino acid residues. Bioinformatics analysis results showed that the predicted molecular quality of PfPDCT protein was about 59.315 ku, with a theoretical isoelectric point of9.48, and the instability coefficient of this protein was 35.00. Phylogeny analysis revealed that PfPDCT was closest to that from Erythranthe guttatus. The expression pattern of PfPDCT gene at different development stages of seeds was analyzed by semi-quantitative PCR. The results showed that the expression level of PfPDCT gene was the highest at 20 d after flowering, which was 1.92 times as much as that of 10 d after flowering. This study will provide a basis for further clarifying the function and mechanism of PfPDCTT gene.

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