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Position: Home > Articles > Dephosphorization of Phytic Acid in Wheat Bran by Phytase from Agrobacterium radiobacter FOOD SCIENCE 2011,32 (22) 105-108

放射型根瘤杆菌植酸酶对麸皮中植酸的脱磷作用

作  者:
李文;王陶;杨克
单  位:
徐州工程学院食品(生物)工程学院
关键词:
植酸酶;脱磷;麸皮
摘  要:
采用放射型根瘤杆菌植酸酶粗酶液水解麸皮中的植酸,研究其脱磷的最适条件。依据单因素试验和正交试验获得脱磷的最优条件为加酶量750U/g、底物质量浓度40mg/mL、温度45℃、pH7、时间5h。最优条件下,麸皮中植酸的水解率在5h达最大,为81.50%。
译  名:
Dephosphorization of Phytic Acid in Wheat Bran by Phytase from Agrobacterium radiobacter
作  者:
LI Wen,WANG Tao,YANG Ke(School of Food Engineering,Xuzhou Institute of Technology,Xuzhou 221000,China)
关键词:
phytase;dephosphorization;wheat bran
摘  要:
Crude phytase solution separated from Agrobacterium radiobacter fermentation broth was used to hydrolyze wheat bran for phytic acid dephosphorization.Using one-factor-at-a-time combined with orthogonal array design method,such process conditions as enzyme amount,substrate concentration,pH,temperature and hydrolysis time were optimized to be 750 U/g,40 mg/mL,7,45 ℃,and 5 h,respectively.Under the optimized conditions,the hydrolysis rate of phytic acid was up to 81.50%.

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