作 者：
葛唯佳;赵岩;图力古尔;李伟;郜玉钢;祝洪艳;李玉;包海鹰
单 位：
吉林农业大学食药用菌教育部工程研究中心;吉林农业大学中药材学院
关键词：
杂色云芝;酸溶性多糖;H22荷瘤小鼠;抗肿瘤;H&E染色
摘 要：
【目的】研究杂色云芝酸溶性多糖对H_(22)荷瘤小鼠的抗肿瘤作用及可能的作用机制。【方法】首先测定杂色云芝酸溶性多糖内中性糖、葡萄糖醛酸及蛋白含量,然后以H_(22)肝癌移植瘤小鼠为模型,设置空白对照组(等量生理盐水)、模型组(等量生理盐水)、环磷酰胺(CTX)组(30 mg/kg)和杂色云芝酸溶性多糖高、中、低剂量组(280,140,70mg/kg),每天给药1次,连续给药10d,比较各组抑瘤率、脏器指数、白细胞介素-6(IL-6)、γ-干扰素(INF-γ)、肿瘤坏死因子-α(TNF-α)、谷丙转氨酶(ALT)、尿素氮(BUN)和肌酐(CRE)等指标;最后在光镜下观察各处理组肿瘤组织H&E染色后的形态变化和细胞凋亡情况,考察杂色云芝酸溶性多糖的抗肿瘤作用及机制。【结果】杂色云芝酸溶性多糖内中性糖含量为656mg/g,葡萄糖醛酸含量为38mg/g,蛋白含量为18mg/g。CTX组和杂色云芝酸溶性多糖高、中、低剂量组均能不同程度抑制肿瘤细胞增殖,抑瘤率分别为68.14%,55.56%,47.01%和37.52%;与CTX组相比,杂色云芝酸溶性多糖组改善了免疫器官指数(P<0.01);与模型组相比,杂色云芝酸溶性多糖组改善提高了血清细胞因子IFN-γ、TNF-α水平(P<0.01),降低了血清IL-6、ALT、BUN和CRE水平(P<0.01)。肿瘤组织H&E染色及凋亡检测结果表明,杂色云芝酸溶性多糖可促使肿瘤细胞凋亡。【结论】杂色云芝酸溶性多糖具有抗肿瘤作用,且可能是通过调节免疫能力实现这种作用的。
译 名：
Antitumor effect of acid-soluble polysaccharides from Trametes versicolor(L.) Lloyd in H_(22) tumor-bearing mice
作 者：
GE Weijia;ZHAO Yan;BAU Tolgor;LI Wei;Gao Yugang;ZHU Hongyan;LI Yu;BAO Haiying;College of Chinese Medicinal Meterial,Jilin Agricultural University;Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi,Jilin Agricultural University;
关键词：
Trametes versicolor(L.) L loyd;;acid-soluble polysaccharides;;H22 tumor-bearing mice;;anti-tumor;;H&E staining
摘 要：
【Objective】This paper aimed to study the antitumor effect and possible mechanism of acidsoluble polysaccharides from Trametes versicolor(L.)Lloyd on H_(22) tumor-bearing mice.【Method】The contents of glucose,glucuronic acid and protein of the acid-soluble polysaccharides from Trametes versicolor(L.)Lloyd were determined and several groups including normal group(equal volume of saline),model group,(equal volume of saline),cyclophosphamide(CTX)group(30 mg/kg)as well as high,medium and low dose groups of acid-soluble Polysaccharides fromTrametes versicolor(L.)Lloyd,acid-soluble polysaccharides were set with H_(22) hepatoma transplanted tumor mice as the model.The groups were treated once a day for 10 continuous days,and inhibitory rate,organ indexes of liver,thymus and spleen,and levels of IL-6,IFN-γ,TNF-α,ALT,BUN and CRE were measured and compared.The morphological changes after the H&E staining of tumor tissue and apoptosis were also observed under light microscope.【Result】The content of glucose in the acid-soluble polysaccharides fromTrametes versicolor(L.)Lloyd was 656 mg/g,the content of glucuronic acid was 38 mg/g,and the content of protein was 18 mg/g.CTX group and the high,medium and low groups of acid-soluble polysaccharides from Trametes versicolor(L.)Lloyd inhibited the growth of tumor cells by 68.14%,55.56%,47.01%,and 37.52%.Compared with CTX group,the immune organ index was ameliorated(P<0.01).Compared with the model group,the levels of serum cytokines IFN-γand TNF-αwere increased(P<0.01),while the levels of serum IL-6,ALT,BUN and CRE were decreased(P<0.01).H&E staining of tumor tissue and apoptosis test showed that acid-soluble polysaccharides promoted tumor cell apoptosis.【Conclusion】The acid-soluble polysaccharides fromTrametes versicolor(L.)Lloyd have antitumor effect,and may be through the regulation of immune capacity to achieve this effect.
