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当前位置: 首页 > 文章 > 两种测定杂色云芝胞外多糖方法的比较 河南科技学院学报(自然科学版) 2010,38 (2) 39-43
Position: Home > Articles > Comparison of two methods determining extro-polysaccharide of Coriolus Versicolor Journal of Henan Institute of Science and Technology(Natural Science Edition) 2010,38 (2) 39-43

两种测定杂色云芝胞外多糖方法的比较

作  者：
贾翠英;张玉辉;李兰
单  位：
河南科技学院
关键词：
杂色云芝;胞外多糖;苯酚硫酸法;蒽酮比色法
摘  要：
采用苯酚-硫酸法和蒽酮-比色法两种方法,分别对云芝多糖含量进行了测定,通过精密度实验、稳定性实验和回收率实验,分析考察两种测定方法的优缺点.结果表明,苯酚硫酸法是测定杂色云芝菌胞外多糖较为理想的方法.此方法与蒽酮比色法相比,其精密度更高,测得多糖平均质量浓度为339.32μg/mL,相对标准偏差为1.07%,稳定性略差于蒽酮比色法,其2h内测得的多糖含量相对标准偏差为0.52%,回收率更理想,且回收率平均值为98.04%,相对标准偏差为0.78%.此研究不仅对杂色云芝固体、液体胞外多糖的测定提供参考方法和选择依据,而且对一些食用菌多糖的测定也提供了一定参考和借鉴意义.
译  名：
Comparison of two methods determining extro-polysaccharide of Coriolus Versicolor
作  者：
Jia Cuiying,Zhang Yuhui,Li Lan (Henan Institute of Science and Technology,Xinxiang 453003,China)
关键词：
Coriolus versicolor;;Extro-polysaccharide;;Vitriol-phenol method;;anthrone colorimetry method
摘  要：
Two methods,vitriol-phenol method and anthrone colorimetry method,determining extro-polysaccharide of Coriolus Versicolor were compared in this paper.The experiment of precision,stability and recovery efficiency were carried out,and we also investigated the advantage and disadvantage of these two methods.The results showed that vitriol-phenol method was a better method than anthran-ketone colorimetry method,it does not only present good stability and good precision but also has high recycle rate,which was firstly shown by measuring the average polysaccharide content of 339.32μg/mL with 1.07%of relative standard difference,and secondly shown the measuring results kept high stability with 0.52%of relative standard difference in two hours,and finally shown higher the average recovery efficiency of 98.04%with 0.78%of relative standard difference.It does not only can offer some references and selection for determining intro or extro-polysaccharide of Coriolus versicolor but also can give some use for references of measuring polysaccharide of some mushrooms.

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