当前位置: 首页 > 文章 > 二温式聚合酶链反应鉴别诊断迟缓爱德华氏菌病 水生态学杂志 2009,2 (2) 158-160
Position: Home > Articles > Idntification of Edwarsiella tarda Strains by Two-Temperature PCR Journal of Hydroecology 2009,2 (2) 158-160

二温式聚合酶链反应鉴别诊断迟缓爱德华氏菌病

作  者:
邓显文;谢芝勋;谢丽基;刘加波;谢志勤;庞耀珊;余晓丽
单  位:
广西兽医研究所;广西水产研究所
关键词:
二温式PCR;鉴别;爱德华氏菌;迟钝爱德华氏菌
摘  要:
根据迟缓爱德华氏菌株23SrDNA基因,设计合成一对引物XZE15、XZE16,建立了二温式聚合酶链反应(PCR)鉴别诊断迟缓爱德华氏菌株的技术。特异性试验表明,对3株迟钝爱德华氏菌株进行PCR扩增出与预期大小相一致的284bp的特异性片段,但对3株钻鱼爱德华氏菌及其他对照鱼病病原体核酸模板的PCR扩增不出现任何条带;敏感性试验结果显示,该二温式PCR可以检测到10pg的迟钝爱德华氏菌DNA。
译  名:
Idntification of Edwarsiella tarda Strains by Two-Temperature PCR
作  者:
DENG Xian-wen1,XIE Zhi-xun1,XIE Li-ji1,LIU Jia-bo1,XIE Zhi-qin1,PANG Yao-shan1,YU Xiao-li2(1.Guangxi Veterinary Research Institute,Nanning530001,China;2.Guangxi Fishery Research Institute,Nanning530021,China)
关键词:
two-temperature PCR;identification;Edwardsiella;Edwarsiella tarda
摘  要:
A pair of primer XZE15 and XZE16 was designed and synthesized according to the conserved gene sequences of 23S rDNA of Edwarsiella tarda,and then reaction parameters were optimized to develop two-temperature polymerase chain reaction(PCR)to simultaneously identify E.tarda.The results showed that only of 284pb-long DNA fragment for three E.tarda strains was amplified,and no DNA fragments for three Edwarsiella ictalur strains and other fish pathogenic viruses and bacteria were amplified by this two-temperature PCR.And 10 pg DNA of E.tarda was detected by this two-temperature PCR.

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