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Position: Home > Articles > Determination of Total Number of Colonies by Fluorescence Labeling Method FOOD SCIENCE 2014,35 (20) 131-134

荧光标记法测定菌落总数

作  者:
张永帅;孙俊良;梁新红;李元召
单  位:
河南科技学院食品学院
关键词:
荧光标记;菌落总数;麝香草酚
摘  要:
采用一种新型破膜剂麝香草酚将微生物细胞壁完全破裂,再用碘化丙啶荧光标记微生物核酸或DNA,测定荧光值。建立工作曲线,根据荧光值在工作曲线上查出菌落总数。结果表明,破膜剂麝香草酚的最适浓度为4 mmol/L,本方法测定枯草芽孢杆菌菌落数对应荧光值的相对标准偏差为1.29%,回收率为98.4%。方法准确可靠,而且不需要大量铺平板,简便快速。
译  名:
Determination of Total Number of Colonies by Fluorescence Labeling Method
作  者:
ZHANG Yong-shuai;SUN Jun-liang;LIANG Xin-hong;LI Yuan-zhao;School of Food Science, Henan Institute of Science and Technology;
关键词:
fluorescence labeling;;the total number of colonies;;thymol
摘  要:
After complete disruption of the microbial cell wall with thymol, the nucleic acids or DNAs were labeled with propidium iodide for the detection of fluorescence values. A standard curve was established for extrapolating total number of colonies based on the fluorescence values. The results showed that the optimal thymol concentration for breaking the microbial cell wall was 4 mmol/L. The relative standard deviation of this method for the determination of the fluorescence of B. subtilis colonies was 1.29%, and the recovery rate was 98.4%. This method is accurate, reliable, simple and rapid without the use of a large amount of plates.

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