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当前位置: 首页 > 文章 > 阿魏酸拮抗PM2.5对A549细胞线粒体的损伤作用 食品科学 2017,38 (3) 195-200
Position: Home > Articles > Protective Effect of Ferulic Acid on PM2.5-Induced Mitochondrial Damage in A549 Cells FOOD SCIENCE 2017,38 (3) 195-200

阿魏酸拮抗PM2.5对A549细胞线粒体的损伤作用

作  者：
劳文艳;毕婷婷;周艳丽;陈世杰;赵晓红;刁屹
单  位：
北京联合大学功能食品科学技术研究院
关键词：
阿魏酸;PM2.5;A549细胞;线粒体;损伤;保护作用
摘  要：
研究阿魏酸是否具有拮抗PM2.5致线粒体损伤作用及拮抗机制。实验分为空白对照组、PM2.5(240 μg/m L(损伤组和阿魏酸(80 mmol/L)保护组。通过Mito SOX Red特异性探针和流式细胞仪测定细胞线粒体内活性氧(reactive oxygen species,ROS)的含量;Annexin-V/PI双染色法流式细胞仪检测细胞凋亡率;JC-1染色法流式细胞仪检测细胞线粒体膜电位的变化;活体细胞线粒体膜通道孔荧光检测试剂盒检测细胞线粒体膜通道孔(mitochondrial permeablity transition pore,m PTP)的开放情况;Western blot法检测caspase-3、caspase-9、含锰金属辅基的超氧化物歧化酶(manganese superoxide dismutase,Mn SOD)和过氧化物酶增殖体激活受体γ共激活因子-1α(subunit of peroxisome proliferators-activated receptor-γ-coactivator-1α,PGC-1α)相关蛋白表达量。结果显示,与空白对照组比较,PM2.5损伤组的细胞ROS含量、caspase-3和caspase-9表达量及细胞凋亡率升高,线粒体膜电位和m PTP活性及PGC-1α、Mn SOD的表达量降低;阿魏酸预处理后,显著降低了PM2.5造成的A549细胞ROS的含量、细胞凋亡率及caspase-3和caspase-9表达量的升高,显著升高了PM2.5致A549细胞线粒体的膜电位、m PTP的活性及PGC-1α和Mn SOD表达量的降低。阿魏酸对PM2.5致A549细胞线粒体损伤具有明显的保护作用。
译  名：
Protective Effect of Ferulic Acid on PM2.5-Induced Mitochondrial Damage in A549 Cells
作  者：
LAO Wenyan;BI Tingting;ZHOU Yanli;CHEN Shijie;ZHAO Xiaohong;DIAO Yi;Research Institute for Science and Technology of Functional Foods,Beijing Union University;Key Laboratory of Bioactive Substances and Functional Foods,Beijing Union University;
关键词：
ferulic acid;;PM2;;5;;A549 cells;;mitochondria;;damage;;protective effect
摘  要：
In this study, we examined whether ferulic acid(FA) could protect against PM2.5-induced mitochondrial damage in A549 cells and explored the underlying mechanism. The cells were treated with PM2.5(240 μg/m L) with and without the addition of 80 mmol/L FA. The contents of reactive oxygen species(ROS) were determined by flow cytometry using Mito SOX Red specific probe. Apoptosis was detected by flow cytometry with Annexin-V/PI method. Mitochondrial membrane potential was detected by flow cytometry with JC-1 staining. The activity of mitochondrial membrane channel pore was tested by mitochondrial permeability transition pore(m PTP) kit. The expression of caspase-3, caspase-9, manganese superoxide dismutase(Mn SOD), and peroxisome proliferators-activated receptor-γ-coactivator-1α(FGC-1α) were detected by Western blotting. The results showed that, compared with the blank control group, the level of ROS and the expression of caspase-3 and caspase-9 in mitochondria, as well as the apoptosis rate of A549 cells induced by PM2.5 were increased. The membrane potential and the activity of m PTP in mitochondria of A549 cells induced by PM2.5 were decreased as well as the expression of PGC-1α and Mn SOD. The pre-treatment of FA could prevent the increased mitochondrial ROS, apoptosis rate and expression of caspase-3 and caspase-9, and the decreased mitochondrial membrane potential, m PTP activity, and expression of PGC-1α and Mn SOD caused by PM2.5. Therefore, FA had a significant protective effect on PM2.5-induced mitochondrial damage in A549 cells.

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