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当前位置: 首页 > 文章 > 不同温度下长茎葡萄蕨藻无机碳利用 广东海洋大学学报 2019 (3)
Position: Home > Articles > 不同温度下长茎葡萄蕨藻无机碳利用 Journal of Guangdong Ocean University 2019 (3)

不同温度下长茎葡萄蕨藻无机碳利用

作  者：
黄丹;刘东超;王晓梁;林挺;黄晓健;欧阳兆攀
单  位：
广东海洋大学水产学院
关键词：
长茎葡萄蕨藻;pH补偿点;无机碳利用;温度;抑制剂
摘  要：
[目的]阐明长茎葡萄蕨藻对无机碳的利用方式.[方法]运用pH漂移技术,探索藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻(Caulerpa lentillifera)的pH补偿点的影响,并研究在3种不同温度(20、25、30℃)下,乙酰唑胺(AZ)、乙氧苯丙噻唑磺胺(EZ)、4,4′-二异硫氰基芪2,2′-二磺酸(DIDS)、钒酸盐(Van)4种抑制剂对长茎葡萄蕨藻无机碳的利用影响.[结果]藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻的pH补偿点均无显著性影响(P>0.05),在一定范围内藻体质量或照度越大,达到pH补偿点时间越短,且pH补偿点均大于9.20℃下各抑制剂的抑制作用最大,其次为30℃,25℃最小,但3种温度下各抑制剂的抑制率无显著差别(P> 0.05).4种抑制剂中DIDS对长茎葡萄蕨藻pH补偿点和无机碳利用均无显著影响(P> 0.05),剩余3种抑制剂的抑制作用依次为EZ>AZ>Van.[结论]藻体质量、藻体部位、照度和海水体积对长茎葡萄蕨藻pH补偿点无影响.温度对长茎葡萄蕨藻吸收利用无机碳没有明显影响.长茎葡萄蕨藻能利用HCO3-作为无机碳源进行光合作用.
作  者：
HUANG Dan;LIU Dong-chao;WANG Xiao-liang;LIN Ting;HUANG Xiao-jian;OUYANG Zhao-pan;Fisheries College of Guangdong Ocean University;Engineering Technology Research Center for Algae Breeding and Application of Guangdong Province;
单  位：
HUANG Dan%LIU Dong-chao%WANG Xiao-liang%LIN Ting%HUANG Xiao-jian%OUYANG Zhao-pan%Fisheries College of Guangdong Ocean University%Engineering Technology Research Center for Algae Breeding and Application of Guangdong Province
关键词：
Caulerpa lentillifera;;pH compensation point;;inorganic carbon utilization;;temperature;;inhibitors
摘  要：
【Objective】To evaluate the utilization of inorganic carbon by Caulerpa lentillifera.【Methods】The effects of algal weight, algal body parts, irradiance and seawater volume on pH compensation point were explored by using the pH drift technology, and the effects of four inhibitors acetazolamide(AZ),6-ethoxyzolamide(EZ), 4,4'-diisothiocyano-stilbene-2,2'-disulfonate(DIDS), vanadate(Van)on the utilization of inorganic carbon in C. lentillifera under three different temperatures(20, 25, 30 ℃)were analyzed.【Results】The results showed that algae weight, algal body parts, irradiance, and seawater volume had no significant effect on the pH compensation points(P > 0.05). The greater the weight of the algae or the greater the irradiance within a certain range, the shorter the time required to reach the pH compensation point which is greater than the pH compensation point 9. The inhibitory effect of each inhibitor was highest at 20 ℃, followed by 30 ℃ and 25 ℃, but there was no significant difference between the inhibitors at different temperatures(P > 0.05). Among the four inhibitors, DIDS had no significant effect on the pH compensation point and inorganic carbon utilization of C. lentillifera(P >0.05), and the inhibition effects of the remaining three inhibitors were as follows: EZ>AZ>Van.【Conclusion】The algae weight, algal body parts, irradiance, and seawater volume had no effect on the pH compensation point of C. lentillifera.The temperature had no significant effect on the utilization of inorganic carbon by C. lentillifera, and HCO_3~-can be used as an inorganic carbon source for photosynthesis by C. lentillifera.

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