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Position: Home > Articles > Ex Vitro Rooting and Plantlet Regeneration of Adventitious Shoots from Microshoots of Physocarpus amurensis (Maxim.) Maxim. Journal of Northeast Forestry University 2006,34 (3) 44-46

风箱果组织培养不定芽的试管外生根及植株再生

作  者:
魏晓慧;殷东生;张丽杰;沈海龙;张鹏
单  位:
东北林业大学
关键词:
风箱果;组织培养;不定芽;试管外生根;植株再生
摘  要:
以风箱果幼苗顶芽为外植体,诱导不定芽,通过试管外生根技术建立植株再生体系。结果表明:以MS+BA1.0 mg·L~(-1)+NAA1.0 mg·L~(-1)为诱导培养基,MS+BA0.8 mg·L~(-1)+NAA0.4 mg·L~(-1)为增殖培养基,MS+IBA0.5 mg·L~(-1)+GA_30.3 mg·L~(-1)为壮苗伸长培养基,效果较好;试管外生根以100 mg·L~(-1)NAA处理30 min效果最佳,生根成活率为93.1%。
译  名:
Ex Vitro Rooting and Plantlet Regeneration of Adventitious Shoots from Microshoots of Physocarpus amurensis (Maxim.) Maxim.
作  者:
Wei Xiaohui Yin Dongsheng Zhang Lijie Shen Hailong Zhang Peng (Northeast Forestry University,Harbin 150040,P.R.China)
关键词:
Physocarpus amurensis;;Tissue culture;;Adventitious shoots;;Ex vitro rooting;;Plantlet regeneration
摘  要:
In vitro adventitious shoot was induced from apical bud of Physocarpus amurensis seedlings,and establishment of pla- ntlet regeneration system was studied by ex vitro rooting of the in vitro regenerated microshoots.Results showed that the op- timum culture media were obtained as MS+BA 1.0 mg·L~(-1)+NAA 1.0mg·L~(-1)for apical bud germination,MS+BA 0.8mg·L~(-1)+NAA 0.4 mg·L~(-1) for shoot proliferation,and MS+IBA 0.5 mg·L~(-1)+GA_3 0.3mg·L~(-1)for shoot elon- gation and strengthening.The best treatment for ex vitro rooting was 100 mg·L~(-1)NAA soaked for 30 min,and the rooting and survival rate reached 93.1%.

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