当前位置: 首页 > 文章 > 免疫捕获PCR检测进境苹果果实中梨火疫病菌 植物检疫 2010,24 (1) 13-17
Position: Home > Articles > Detection of Erwinia amylovora in imported apple fruit by immuno-capture PCR. Plant Quarantine 2010,24 (1) 13-17

免疫捕获PCR检测进境苹果果实中梨火疫病菌

作  者:
何丹丹;周国梁;陈仲兵;印丽萍;徐殿胜;易建平
单  位:
上海出入境检验检疫局;华东理工大学生物工程学院
关键词:
梨火疫病菌;免疫捕获PCR;苹果
摘  要:
利用梨火疫病菌抗体和PCR技术建立了梨火疫病菌免疫捕获PCR方法,检测苹果模拟样品的灵敏度达到了1.5×102cfu/reaction。与直接PCR进行比较,免疫捕获PCR方法检测苹果模拟样品的灵敏度提高了10倍以上,而且省去了DNA提取等步骤。利用该方法成功从进境苹果样品中检测到梨火疫病菌,产物测序结果表明,产物序列和梨火疫病菌的相应序列高度一致。试验结果表明,免疫捕获PCR法能除去样品中的大部分PCR反应抑制物质,可以有效检测进境苹果中梨火疫病菌,在口岸水果检疫中具有一定的应用潜力和推广价值。
译  名:
Detection of Erwinia amylovora in imported apple fruit by immuno-capture PCR.
作  者:
He Dandan1,Zhou Guoliang2,Chen Zhongbing2,Yin Liping2,Xu Diansheng1,Yi Jianping2*(1.East China University of Science and Technology,Shanghai 200237,China;2.Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China)
关键词:
Erwinia amylovora;immuno-capture PCR;detection
摘  要:
The immuno-capture polymerase chain reaction(IC-PCR) technique by combining immuno-capture of the bacteria and polymerase chain reaction(PCR) amplification of its amsG gene was developed for direct detection of Erwinia amylovora in imported apple fruits with the sensitivity of the detection as low as 150 cfu in artificial contaminated samples.This level of detection sensitivity of at least 10 fold over direct PCR was confirmed in artificial apple samples with the advantage of not requiring DNA extraction steps while still allowing an accurate diagnosis of Erwinia amylovora.The successful detection for Erwinia amylovora in imported apple sample indicated that this IC-PCR assay is suitable for the large-scale screening Erwinia amylovora with a great potential to be used for routine apple sample assay.

相似文章

计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊