中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 不同长度的聚肌胞对SPF鸡固有免疫增强作用的研究 中国预防兽医学报 2017 (11) 932-935
Position: Home > Articles > Research on Poly I:C of different lengths as immunopotentiator for specific-pathogen-free chickens Chinese Journal of Preventive Veterinary Medicine 2017 (11) 932-935

不同长度的聚肌胞对SPF鸡固有免疫增强作用的研究

作  者：
黄艳艳;魏冉;张琳;刘俊珍;高丹丹;崔言顺;张秀美;吴家强
关键词：
聚肌胞;鸡;固有免疫;免疫增强剂
摘  要：
聚肌胞(poly I:C,p IC)作为强干扰素诱生剂,在人类医学上被用作抗病毒和抗肿瘤药物。已有的研究表明,聚合长度不同的p IC对哺乳动物细胞的免疫增强作用不同。为明确p IC的聚合长度是否会影响其对鸡的固有免疫增强作用,本研究将低分子量p IC(0.3 kb~1 kb)和高分子量p IC(6 kb~8 kb)分别肌肉注射1月龄的SPF鸡,各设置2个p IC浓度梯度。在p IC作用后的6 h、12 h、24 h和36 h,分别采集EDTA抗凝血,分离外周血淋巴细胞(PBL),通过荧光定量PCR方法检测PBL固有免疫信号通路相关基因的转录水平变化。结果表明,低和高分子量范围的p IC刺激SPF鸡后均可以迅速激活鸡体固有免疫应答,引起TLR3、MDA5、IFN-α、IFN-β、IFN-γ、IL-1β、IL-2、IL-6和IL-17等免疫相关基因转录水平的上调。该结果与哺乳动物模型的研究报道截然不同,而与本实验室以多种鸡源细胞为模型的研究结果相似。10μg/kg(质量/体质量)的p IC即可以引起PBL固有免疫相关基因转录水平上调,而且大部分基因在p IC刺激后6 h即达到转录峰值,表明p IC作为鸡用免疫增强剂的高效性。总之,本研究表明短链和长链p IC均可用作鸡免疫增强剂。
译  名：
Research on Poly I:C of different lengths as immunopotentiator for specific-pathogen-free chickens
作  者：
HUANG Yan-yan;WEI Ran;ZHANG Lin;LIU Jun-zhen;GAO Dan-dan;CUI Yan-shun;ZHANG Xiu-mei;WU Jia-qiang;Animal Husbandry and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong Key Laboratory of Animal Disease Control and Breeding;College of Animal Science, Shandong Agricultural University;
单  位：
Animal Husbandry and Veterinary Medicine, Shandong Academy of Agricultural Sciences, Shandong Key Laboratory of Animal Disease Control and Breeding%College of Animal Science, Shandong Agricultural University
关键词：
poly I:C;;chicken;;innate immunity;;immnopotentiattor
摘  要：
Poly I:C(p IC) is a strong interferon inducer, and is used as an anti-viral/tumor drug in human medicine. Recent research has found distinct function of p ICs with different molecular lengths on mammalian cells. It is unclear whether the length of p IC is also a key factor that influences the immune function in chicken. In this study, low(0.3 kb-1 kb) and high-molecularweight(6 kb-8 kb) p IC were employed to inject specific-pathogen-free chickens through muscular route. At 6, 12, 24 and 36 hrs post stimulation, EDTA-anticoagulating blood was collected and peripheral blood lymphocytes(PBLs) were separated from the blood. Real-time PCR assays were used to detect the expression dynamics of PBL cellular innate immune-related genes. The results showed that p IC of both low and high molecular weights could quickly boost chicken cellular innate immune response,resulting in the up-regulation of innate immunity-related genes including TLR3, MDA5, IFN-α, IFN-β, IFN-γ, IL-1β, IL-2, IL-6 and IL-17. The result was distinct from that of mammalian models, while similar to that of chicken cellular models(unreported data from our research). In particular, p IC of concentration(mass/body weight) as low as 10 μg/kg resulted in significant up-regulation of chicken immune-related genes with most genes reaching transcriptional peaks at 6 hs post stimulating, which indicated the efficacy of p IC as chicken immunopotentiator. In conclusion, this study showed the prospection of p ICs of both low and high molecular lengths as chicken immunopotentiator.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46