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当前位置: 首页 > 文章 > 植物乳杆菌分子伴侣蛋白基因在盐胁迫下的表达分析 食品科学 2015,36 (11) 95-99
Position: Home > Articles > Expression of Chaperone Protein Genes in Lactobacillus plantarum under Salt Stress FOOD SCIENCE 2015,36 (11) 95-99

植物乳杆菌分子伴侣蛋白基因在盐胁迫下的表达分析

作  者：
乌日娜;宋雪飞;刘倩颖;徐鑫;王茜茜;武俊瑞
单  位：
沈阳农业大学食品学院
关键词：
植物乳杆菌;实时荧光定量聚合酶链式反应;盐胁迫;基因表达
摘  要：
以一株分离筛选自东北自然发酵大酱中的耐盐植物乳杆菌FS5-5为实验对象,通过实时荧光定量聚合酶链式反应技术,在转录水平上对其分子伴侣蛋白的相应基因在盐胁迫下的表达进行研究。结果表明:在菌体对数生长期,分子伴侣蛋白调控系统中,基因gro EL、gro ES、dna K、dna J、hsp1、hsp2、usp均受MRS培养基中Na Cl的诱导而表达上调,并且Na Cl的质量浓度越高,基因受诱导表达上调越显著,而hsp3虽受MRS培养基中Na Cl的诱导表达有所上调,但其受诱导表达上调显著程度与Na Cl质量浓度不呈正相关。
译  名：
Expression of Chaperone Protein Genes in Lactobacillus plantarum under Salt Stress
作  者：
WU Rina;SONG Xuefei;LIU Qianying;XU Xin;WANG Qianqian;WU Junrui;College of Food Science,Shenyang Agricultural University;State Key Laboratory of Food Science and Technology,School of Food Science and Technology,Jiangnan University;
关键词：
Lactobacillus plantarum;;real-time fluorescence quantitative polymerase chain reaction(real time-PCR);;salt stress;;gene expression
摘  要：
The objective of this study was to isolate a salt-tolerant Lactobacillus plantarum(FS5-5) from naturally fermented miso in northeastern China.The expression of chaperone protein genes under salt stress at the transcriptional level was evaluated by real-time fluorescence quantitative polymerase chain reaction(real time-PCR).Results showed that in the exponential growth phase,the chaperone protein genes such as gro EL,gro ES,dna K,dna J,hsp1,hsp2,and usp were induced by Na Cl in MRS medium and their expressions were up-regulated.The higher Na Cl concentration was,the more significant the up-regulation was.Similarly,the heat shock protein gene hsp3 was induced by Na Cl in MRS medium and its expression was up-regulated,but the induced up-regulation was not positively correlated with Na Cl concentration.

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