当前位置: 首页 > 文章 > 北京油鸡和矮脚鸡心脏型、脂肪型脂肪酸结合蛋白基因多态性的厂家 畜牧兽医学报 2003,34 (5) 422-426
Position: Home > Articles > RFLPs at Heart and Adipocyte Fatty Acid Binding Protein Genes in Beijing Oil Chick and Aijiao Chick Chinese Journal of Animal and Veterinary Sciences 2003,34 (5) 422-426

北京油鸡和矮脚鸡心脏型、脂肪型脂肪酸结合蛋白基因多态性的厂家

作  者:
叶满红;曹红鹤;文杰;李宏滨;陈继兰;赵桂萍
单  位:
中国农业科学院畜牧研究所
关键词:
H-FABP;A-FABP;PCR-RFLP;鸡
摘  要:
根据不同物种间同一基因核苷酸序列的保守性及相似性,设计特异性引物,利用聚合酶链式反应技术(PCR),扩增鸡心脏型脂肪酸结合蛋白(H—FABP)基因第二内含子序列;根据已经发表的鸡脂肪型脂肪酸结合蛋白(A-FABP)基因mRNA序列,设计特异性引物,扩增鸡A-FABP基因第三内含子序列。选用Hha I、HaeIII、Hinf I、BamH I、EcoR I、Pst I、Xmn I和Hind IIi 8种限制性内切酶对扩增产物分别进行单酶切,发现H-FABP基因第二内含子Hha I PCR-RFLP及A-FABP基因第三内含子Hinf I PCR-RFLP。
译  名:
RFLPs at Heart and Adipocyte Fatty Acid Binding Protein Genes in Beijing Oil Chick and Aijiao Chick
作  者:
叶满红(1);;曹红鹤(1);;文杰(1);;李宏滨(1);;陈继兰(1);;赵桂萍(1)
单  位:
(1)中国农业科学院畜牧研究所, 北京, 100094
摘  要:
Specific primer was designed to amplify the second intron of H-FABP gene in Beijing Oil Chick accord-ing to the conservative sequences of H-FABP genes from different species. We also designed another specific primer to amplify the third intron of A-FABP gene in Beijing Oil Chick. Hha I , Hae III , Hinf I , BamH I , EcoR I , Pst I , Xmn I and Hind HI were used to digest PCR products respectively. Two Polymerase Chain Re-action-Restriction Fragment Length Polymorphisms (PCR-RFLPs) were found, polymorphic Hha I site in H-FABP and polymorphic Hinf I site in A-FABP.

相似文章

计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊