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Position: Home > Articles > Study on Tissue Culture and Plantlet Regeneration from Stemlet of Faba Bean Modern Agricultural Science and Technology 2010 (19) 104+108

春蚕豆茎段组织培养和植株再生研究

作  者:
彭绣科;刘洋
单  位:
青海省农林科学院
关键词:
春蚕豆;茎段;组织培养;植株再生
摘  要:
以青海当地主栽品种青海11号的茎段为外植体,探讨了不同生长调节物质、不同糖浓度对外植体分化的影响以及外植体不同苗龄的分化能力差异,其中以添加2.0~3.0 mg/L 6-BA、0.05~0.08 mg/L NAA的培养基诱导芽的分化效果最好,且越幼嫩的外植体诱导芽分化越容易,并建立了青海11号茎段的高效再生体系。
译  名:
Study on Tissue Culture and Plantlet Regeneration from Stemlet of Faba Bean
作  者:
PENG Xiu-ke LIU Yang(Qinghai Academy of Agricultural and Forestry Sciences,Xining Qinghai 810016)
关键词:
spring faba bean;stemlet;tissue culture;plant regeneration
摘  要:
Using stemlet of major cultivar(Qinghai NO.11)of faba bean,the influence of different growth regulating substances,different sugar concentration and ages on explant differentiation were studied.The culture media added 2.0~3.0 mg/L 6-BA,0.05~0.08 mg/L NAA showed the best differentiation effect,and the younger and tenderer the explant was,the easier the differentiation of inducing bud was.Then high efficient regeneration system of Qinghai NO.11 was established.

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