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当前位置: 首页 > 文章 > 软枣猕猴桃果胶降解工艺优化及降解产物抗氧化活性研究 沈阳农业大学学报 2019 (2) 167-173
Position: Home > Articles > Optimization of Pectin Degradation Process and Antioxidant Activity of Degradation Products of Actinidiadeliciosa Journal of Shenyang Agricultural University 2019 (2) 167-173

软枣猕猴桃果胶降解工艺优化及降解产物抗氧化活性研究

作  者：
康振宇;吴优;赵奕彭;邢尧;康宗利;杨玉红
单  位：
沈阳农业大学生物科学与技术学院
关键词：
软枣猕猴桃;果胶降解;响应面优化;抗氧化
摘  要：
为研究软枣猕猴桃果胶降解产物的活性,选用软枣猕猴桃为原料,通过响应面设计对果胶降解工艺进行优化,利用抗氧化试验对生物活性进行研究。选取料液比、果胶酶活力、果胶降解时间及果胶降解温度4个影响因素,进行四因素三水平响应面试验。通过响应面优化得到软枣猕猴桃果胶降解的最佳条件组合为:料液比为1∶5g·mL-1、果胶酶活力为5000U·g-1、果胶的降解时间为2.5h、果胶的降解温度为45℃,此条件下果胶降解产物的降解率为57.62%。抗氧化活性试验结果表明软枣猕猴桃果胶的降解产物对DPPH自由基清除能力为(70.14±1.23)%,对超氧阴离子自由基清除能力为(55.39±1.82)%,对烷基自由基清除能力为(53.98±1.95)%,还原力可达1.229±0.14。响应面优化的降解工艺切实可行,软枣猕猴桃果胶的降解产物具有一定的抗氧化活性。
译  名：
Optimization of Pectin Degradation Process and Antioxidant Activity of Degradation Products of Actinidiadeliciosa
作  者：
KANG Zhen-yu;WU You;ZHAO Yi-peng;XING Yao;KANG Zong-li;YANG Yu-hong;College of Bioscience and Biotechnology, Shenyang Agricultural University;
关键词：
Actinidiadeliciosa;;pectin degradation;;response surface optimization;;antioxidant
摘  要：
In order to study the activity of pectin degradation products of Actinidiaarguta, the Actinidiaarguta was used as raw material, and the degradation process of pectin was optimized by response surface design. The biological activity was studied by anti-oxidation test. Four factors including the ratio of material to liquid, the activity of pectinase, the degradation time of pectin and the degradation temperature of pectin were selected to carry out four-factors three levels response surface test. The optimum conditions for the degradation of pectin in Actinidiaarguta were obtained by response surface optimization were as follows, the ratio of material to liquid was 1:5 g·mL-1, the activity of pectinase was 5000 U·g-1, and the degradation time of pectin was 2.5 h.The degradation temperature of pectin was 45°C. Under this condition, the degradation rate of pectin degradation products was57.62%. The results of antioxidant activity test showed that the degradation of DPPH free radicals by the degradation of Actinidiaarguta pectin was(70.14±1.23)%, and the scavenging ability of superoxide anion radicals was(55.39±1.82)%. The capacity is(53.98±1.95)% and the reducing power is 1.229±0.14. The degradation process optimized by response surface is feasible. The degradation products of Actinidiaarguta pectin have certain antioxidant activity.

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